Bacterial vaginosis in Khartoum State, Sudan: Comparison of Gram stain with Pap test procedures
April 2017
The prime purpose of this study was to define the reliability of the Pap test in establishing the diagnosis of bacterial vaginosis (BV) as well as ascertaining the trustworthiness of Pap test and Gram stain in the diagnosis of bacterial vaginosis using Amsel’s criteria as a gold standard method. Thereby prospective study conducted included 300 females attending to the Department of Gynecology and Obstetrics in our...
Evaluation of microbial adverse effects on fresh and processed bovine meat in N'Djamena (Chad) and Yaoundé (Cameroun)
April 2017
A study from May 2015 to February 2016 was conducted in N'Djamena and Yaoundé regions to assess the microbial hazards associated with fresh and transformed bovine meat. A total of 120 samples of dried, fresh and spicy dry meat (kilichi) were collected. The microbiological results showed that 70% of dried meat from N’Djamena had Escherichia coli as major contaminant compared to 55% of dried meat from...
Prevalence and antibiotics resistance patterns of Salmonella isolated from kitchen sponges at Jimma town, Ethiopia
April 2017
It is identified that through the cleanout practice of utensils, dishes, etc. in kitchens, the before washing and after washing activities are done with the use of sponges to remove food remains. These food residues along with the wetness in the sponges tender an encouraging environment for microbial proliferation. Sponges and tea towels used in cleaning equipments and utensils have been known as possible agents in the...
Association of N2-fixing cyanobacteria with wheat (Triticum vulgare L.) roots
April 2017
Two locally prevailing hetyerocystous cyanobacterial isolates, collected from alluvial clay soil and identified as Nostoc muscorum and Anabaena variabilis, were tested (either singly or mixed) for their potency to colonize the roots of wheat seedlings. N. muscorum formed close association with root hairs, whereas A. variabilis showed loose binding. The mixture of both cyanobacterial strains formed weak association with...
Screening of Lactobacillus spp. from raw goat milk showing probiotic activities against pathogenic bacteria
April 2017
This research work was executed to isolate potential Lactobacillus spp. from local raw goat milk samples for their antimicrobial activities against different human intestinal pathogens. Isolation of such bacteria was carried out by Man Rogosa Sharpe (MRS) agar media. Identification of the isolated strain was conducted according to the morphological and biochemical tests. Furthermore, growth optimization and their...
Comparative study for growth and sporulation of some mycotoxigenic fungi in relation to water activity effects
April 2017
This study examined the effect of water activity (0.85-0.995 aw) on growth rate and asexual spore production for four mycotoxigenic strains (Aspergillus flavus, Aspergillus ochraceus, Aspergillus carbonarius and Penicillium verrucosum) on Malt Extract Agar (MEA). The water activity levels of MEA media were modified ionically (NaCl) and non-ionically (glycerol). Results showed that the optimum aw for growth was at...
Evaluation of banana genotype resistant to Xanthomonas wilts disease (Xanthomonas campestris pv. musacearum) in south east of Ethiopia
April 2017
Xanthomonas wilt caused by Xanthomonas campestris pv.musacearum (Xcm) is one of the most important constraints to banana production. The use of resistant banana varieties would be a long-term and cost-effective solution to control any pathogen. Therefore, identifying this pathogen resistant banana genotype is one of the basic requirements for effective management. The current study was therefore initiated to evaluate...
Identification of acetic acid bacteria isolated from Tunisian palm sap
April 2017
Date palm sap (Phoenix dactylifera L.), called Lagmi, is a sugary substrate that readily ferments through the activity of native microflora that consists mainly of yeasts, lactic acid bacteria and acetic acid bacteria (AAB). The aim of this work was to perform a preliminary step in the isolation and identification of AAB species from different samples of palm sap collected in Southern Tunisia, in order to use them as a...
Isolation of gut associated bacteria from mangrove crabs collected from different mangrove regions of Tamil Nadu, South east coast of India
April 2017
Mangrove crabs are mostly herbivorous which consume more amount of leaf litter of various mangrove species and also plays most important role in leaf degradation. Several studies reported that crab harbor bacteria from the environment through water and food. Bacterial species of the gut can influence the health and robustness of the host. The present study aims to isolate and enumerate the bacterial count from the gut...
Microbiological quality and safety of some-street-vended foods in Jimma Town, Southwestern Ethiopia
April 2017
Street food vending has been benefiting both consumers, who are the in low socio economic status, as well as vendors, by creating job opportunities. However, street foods are perceived to be a major public health risk due to contamination. The objective of this study was to evaluate the microbiological quality and safety of street-vended foods in Jimma town in Ethiopia. The study involved collection of socio-economic...
Microbiological study of cases of early neonatal sepsis and evaluation of the role of C-reactive protein, interleukin-6 and interleukin-8 as diagnostic biomarkers of such cases
April 2017
Neonatal sepsis is responsible for significant morbidity and mortality. Its early diagnosis is very important but very difficult. This study aimed to evaluate the value of C reactive protein (CRP), interleukin 6 (IL-6) and interleukin 8 (IL-8) as early diagnostic biomarkers for early neonatal sepsis (ENOS). Forty neonates with prenatal risk for neonatal sepsis with their mother were taken in this study (group I). Ten...
Potential of Streptomyces sp. and Trichoderma sp. as compost microbiota for coffee husk
April 2017
About 70 to 80% of the coffee berries husk end up as agricultural waste after processing. Its neglect or improper utility can cause environmental pollution. The present study investigated the efficiency of Streptomyces, Trichoderma harzianum and the combination of both as bio-degradable agents of coffee husk which is regarded as agricultural waste. Identification of their efficiency is necessary to stimulate further...
Probiotic potential Streptomyces species from the grains of pearl millet (Pennisetum glaucum)
April 2017
Actinomycetes have been demonstrated for plant growth-promotion, antagonistic against plant pathogens and insect pests and biofortification traits in many agriculturally important crops. The present investigation was conducted to characterize probiotic properties of actinomycete(s) isolated from pearl millet flour and batter samples. A media selective and specific were used for isolation, actinomycetes isolation agar...
Riboflavin enriched iru: A fermented vegetable protein
April 2017
African locust bean (Parkia biglobosa) cotyledon is fermented in most countries of West Africa to produce a soup condiment, known as ‘iru’ in Yoruba language, or ‘dawadawa’ in the predominant Hausa language. Iru is rich in minerals and serves as a source of protein supplement in the diet of poor families. Riboflavin (Vitamin B2) is an essential component of basic cellular metabolism but its...
Isolation and characterization of enteropathogenic and enterotoxinogenic Escherichia coli from dairy products consumed in Burkina Faso
April 2017
Food-borne diseases represent a public major health problem, and drink-water, juice, meat, and milk products are usually involved. This study aimed to evaluate the antibiotic resistance of diarrheagenic E. coli isolated from dairy products consumed in Burkina Faso. Five hundred and twenty-two samples were gathered. Escherichia coli were isolated using Standard Microbiological Methods. A 16-plex polymerase chain reaction...
A cross sectional study on Salmonella in apparently healthy sheep and goats slaughtered at Elfora and Luna export abattoirs, Ethiopia
April 2017
A cross-sectional study was conducted between November 2015 and March 2016 on apparently healthy slaughtered sheep and goats, and clean knife at Luna and Elfora export abattoirs located at Modjo and Bishoftu towns to estimate the prevalence of Salmonella in sheep and goats, to assess the hygienic condition of flaying knife, and to isolate and identify the prevalent Salmonella sub-species. A total of 525 samples...
Toxigenic Staphylococcus aureus in processing of coalho and mozzarella cheese
April 2017
Staphylococcus aureus is commonly involved in food poisoning due to production of toxins responsible for causing animal and human diseases. In this study, 60 strains of presumptive S. aureus isolates from raw milk and cheese were biochemically identified in four dairies: 54 (90%) from refrigerated raw milk (RRM) with counts exceeding 106 CFU/mL, and six (10%) from cheese with similar concentrations of CFU/mL. Out of the...
In silico analysis of Chikungunya virus (CHIKV), a mosquito-borne alphavirus
March 2017
Chikungunya virus (CHIKV) is a mosquito-borne alpha virus, which belongs to the family, Togaviridae. This virus is known to cause an acute onset of high fever, severe arthralgia and rash, and is usually accompanied by headache and severe joint pain. The present study aimed to construct an updated phylogenetic tree of currently published data and perform a phylogeographic analysis of Chikungunya virus obtained during...
Fungal diversity and community structure in gut, mound and surrounding soil of fungus-cultivating termites
March 2017
The fungus-cultivating termites (Macrotermitinae) form part of diverse termite fauna in Africa, but information on their fungal symbionts is inadequate and poorly understood. In this study, the fungal communities and structure between termite gut, mound and surrounding soil were determined using the 454 pyrosequencing-based analysis of the internal transcribed spacer (ITS) gene sequences. Genomic DNA was extracted and...
Antibacterial effects of Anogeissus leiocarpus (DC.) Guill. & Perr. and Terminalia glaucescens Planch. Ex Benth. on rapidly growing mycobacteria species
March 2017
The development of effective and less toxic antimicrobial agents is required for the treatment of respiratory tract infections. This study was carried out to evaluate the phytochemical and antibacterial activities of Anogeissus leiocarpus (DC.) (Guill. & Perr.) and Terminalia glaucescens (Planch. ex Benth.) against non-tuberculous mycobacteria species. The methanol, dichloromethane and aqueous extracts were screened...
Phenotypic and genotypic detection of macrolide-lincosamide-streptogramin B resistance among clinical isolates of Staphylococcus aureus from Mansoura University Children Hospital, Egypt
March 2017
Widespread use of Macrolide, lincosamide and Streptogramin B (MLSB) family of antibiotics in the treatment of Staphylococcus aureus (S. aureus) has led to an increased resistance to MLSB antibiotics. The purpose of this study was to determine the frequency of MLSB resistance among clinical isolates of methicillin sensitive S. aureus (MSSA) and Methicillin resistant S. aureus (MRSA) from Mansoura University Children...
Isolation and characterization of potential probiotic enterococci strains from soft cheese flora
March 2017
This study was conducted to determine the in-vitro probiotic properties of Enterococcus faecium strains isolated from soft cheese. To evaluate the safety of Enterococcus strains, we compared the pathogenic genes, antimicrobial susceptibility of the probiotic strains to those of clinical isolates, and their antimicrobial activity against food-borne pathogenic and spoilage bacteria. Enterococcus strains were identified...
Mycoflora associated with the goat’s hair and sheep wool in Taif, Saudi Arabia
March 2017
The objective of this study was to evaluate the occurrence of mycoflora in 30 samples of healthy goat’s hair and sheep wool collected from different localities in Taif, Saudi Arabia, and the ability of some fungal isolates for keratinase activities. Sixty four species belonging to 28 genera were collected from the two substrates. The wool of sheep was polluted with fungi than goat hairs, and contained high total...
Microbiological evaluation and development of quality index method (QIM) scheme for farmed pintado fish (Pseudoplatystoma corruscans)
March 2017
Experiments were conducted to evaluate the microbiological quality and develop a quality index method (QIM) scheme for farmed pintado fish (Pseudoplatystoma corruscans), with estimation of shelf life. Microbiological analyses showed that two samples of pintado fish were unacceptable for human consumption due to the presence of Salmonella spp. The presence of Staphylococcus aureus in all six samples of pintado can be...
Antibiotic resistance patterns of lactic acid bacteria isolated from Nigerian grown salad vegetables
March 2017
The reports on some foodborne lactic acid bacteria (LAB) possessing antibiotic resistance (AR) genes on mobile genetic elements are on the increase. In Nigeria, such information is rare. This study was therefore designed to determine the presence and locations of AR genes in LAB isolated from locally grown salad vegetables. The LAB used in this study were previously isolated from Nigerian grown cabbage, carrot, cucumber...
Enhanced production of alkaline protease by Aspergillus niger DEF 1 isolated from dairy form effluent and determination of its fibrinolytic ability
March 2017
Proteases constitute most important enzymes owing to their wide variety of functions and have immense applications in various industries viz., medical, pharmaceutical, biotechnology, leather, detergent, and food industries. Despite of their wide spread occurrence in various sources, microorganisms present remarkable potential for proteolytic enzymes production due to their extensive biochemical diversity and...
Combined efficacy of thymol and silver nanoparticles against Staphylococcus aureus
March 2017
Due to the looming spread of resistance to classical antimicrobial agents, innovative therapeutic methods are in dire need to combat the onslaught of resistant bacterial pathogens. This study examines the antimicrobial efficacy of a phytochemical and a metallic nanoparticle against the top Gram positive resistant pathogen. The potential synergy of these two agents was also evaluated. The antibacterial activity of thymol...
Evaluation of safety and immunogenicity of inactivated whole culture contagious caprine pleuropneumonia trial vaccine in National Veterinary Institute, Ethiopia
March 2017
Contagious caprine pleuropneumonia (CCPP) is a fatal disease of goats occurring in many countries of Africa and Asia where the total goat population is more than 500 million. Vaccination is the most cost effective technique in the control of CCPP than any other control measures. In National Veterinary Institute (NVI) inactivated mycoplasma protein based vaccine obtained by centrifugation has been in use since many...
Molecular identification and characterization of Salmonella species isolated from poultry value chains of Gazipur and Tangail districts of Bangladesh
March 2017
The aim of this study was to identify and characterize Salmonella species isolated from poultry value chains of Gazipur and Tangail districts of Bangladesh during the period of October 2015 to May 2016. For this purpose a total of 153 samples (35 chick meconium, 49 cloacal swab, 30 poultry carcass, 14 feed, 16 water, 7 transport swab, 2 floor swab) were collected and were subjected to various cultural, biochemical...
Use of clinical clue to diagnose anaerobic oral and maxillofacial infections among patients at Muhimbili National Hospital, Dar-es-Salaam, Tanzania
March 2017
Increase in life threatening oral facial infection despite the use of antimicrobial among patients attending muhimbili national Hospital, Tanzania has made it imperative to investigate bacteria causing infections. However, comprehensive anaerobic bacteriology of clinical specimens is expensive and time consuming procedure. This study aim therefore at providing information on the use of clinical clue, to diagnose...
Characteristics of cellulase in cellulose-degrading bacterium strain Clostridium straminisolvens (CSK1)
March 2017
Microbial degradation of biomass was considered as a clean and highly efficient approach to produce bioenergy, as it could mitigate the urgent demand for limited petroleum and natural gas. A thermophilic microbial consortium (MC1) was proved a high efficiency cellulose-degrading bacterial community in previous studies. A novel anaerobic, thermophilic, and cellulolytic bacterium (strain CSK1) was isolated from MC1. The...
Probiotic profiling of Leuconostoc species isolated from a traditional fermented cassava product
March 2017
The various properties of lactic acid bacteria have made them good auxiliary in the manufacturing process in agro food industries and farms. They are widely used as probiotics which can be defined as living microorganisms that have beneficial effects on human health probiotics could replace antibiotic growth promoters in livestock without creating new threats such as that observed with antibiotics. Before their use as...
Degradation of diesel-oil by a newly isolated Kocuria sediminis DDK6
March 2017
A bacterial strain named DDK6 was isolated from diesel-contaminated soil from a petrol station in Al-Hofuf city, after enrichment on diesel oil. The strain DDK6 formed a reddish-pink colony with a 2 to 3 mm diameter after two days of incubation at 30°C. Cells were Gram-positive coccoid and formed no endospores. Phenotypic identification by the automated bacterial system, Vitek compact II, identified the DDK6 as...
Inhibition of aflatoxin B1 production of Aspergillus flavus isolated from peanut seeds using Lycium halimifolium Mill leaves fractions
March 2017
The aim of this study is to explore the impact of Lycium halimifolium Mill leaves extracts on mycotoxin B1 production in vitro, as well as the inhibitor effect of the aflatoxin B1 synthesis by Aspergillus flavus strain. The strain was isolated, identified (gi |146746162 | EF409803.1.) and positively tested for the production of Aflatoxin B1. The antimycotoxinogenesis effect was investigated using L. halimifolium Mill...
In vitro cytotoxicity and biological activities of Genipa americana (Rubiaceae) ethanolic extracts
March 2017
This study aimed to determine the in vitro toxicity of Genipa americana and its antiviral and anti-microbial activities. Due to lack of knowledge on G. americana validation as medicinal plant, biological studies were performed. For the cytotoxicity experiments, decreasing concentrations of ethanolic extracts of G. americana branches, fruits, and leaves were added to a confluent monolayer of Vero cells. After 72 h, it...
Effect of tobacco tar on Staphylococcus aureus and Candida albicans biofilm formation
March 2017
Twenty compounds were determined in the tar of cigarette smoke. The tar was obtained using tobacco filters and then analyzed by liquid chromatography-mass spectrometry (LC-MS). Tobacco tar expressed marked decrease in the susceptibility of Staphylococcus aureus to penicillin, tetracycline and amoxicillin/clavulanic antibiotics and showed marked decrease in susceptibility of Candida albicans to the tested antifungals...
Activity of metabolites produced by new strains of Lactobacillus in modified de Man, Rogosa and Sharpe (MRS) medium against multidrug-resistant bacteria
February 2017
The emergence of nearly untreatable infections caused by multidrug-resistant bacteria has led to a new public health concern in which a need for development of alternative non-antibiotic strategies has become urgent. The activity of metabolites produced by new strains of Lactobacillus against multidrug-resistant bacteria was investigated. The objective of this work was to isolate and identify lactobacilli from artisanal...
Effect of nutritional parameters and NaCl concentration on phosphate solubilization potential of Penicillium purpurogenum Stoll isolated from paddy field
February 2017
Phosphorus (P) is the major essential macronutrient of plants. But its availability in Indian soil is relatively low due to high rate of P fixation. The use of phosphate solubilizing microorganism (PSM) to solubilize the fixed form of P is economically reasonable and ecologically safe as compared to chemical phosphatic fertilizers. Fungi have been reported to possess greater ability to solubilize insoluble phosphate...
Screening of biologically active constituents from leaves of Aloe elegans and their antimicrobial activities against clinical pathogens
February 2017
The flourishing to the war b/n pathogens and antibiotics is contingent on the diligent trial to find effective antibiotics; so that to lessen the fear faced by resistant microbes. The screening carried out manifested the presence of saponins, glycosides, tannins, reduced sugars, terpenoids, flavonoids and phenols. Alkaloids and steroids were not traced from any of the extracts. Aqueous and solvent extracts from...
Antibiotic potentials and isolation of metabolomes from microorganisms of mesophilic soil of Rajasthan, India
February 2017
The present of research work was focused on mesophilic soil samples from four different sites of Jaipur (The Pink City) in Rajasthan. Rajasthan is a semi arid region and famous for its sandy soil, heat and temperature which reach up to 50°C in summers. Twenty nine colonies were isolated from the soil samples on nutrient agar media and three distinct isolates were selected and characterized after analyzing coloration...
Antimicrobial activity of some lactic acid bacteria isolated from local environment in Egypt
February 2017
The aims of this study were to investigate antimicrobial activities of what so-called wild strains of LAB isolated from different sources of Egyptian environment including raw and fermented milk, cheeses, other dairy products, sausage and silage. Isolation of lactobacilli and lactococci were carried out onto plates of MRS and M17 agar, respectively, grouped to identification and the obtained treated supernatants were...
Microorganisms in functional food supplementation: A review
February 2017
Use of microbial systems for functional foods supplementation can be defined into fermentative process by starter cultures, use of probiotics microorganisms and use of microbial biomass. Scientific and technical aspects as related to use of microorganisms in functional foods supplementation are highly diverse and complex, since they have to deal with the viability of probiotic strains as well as with the safe...
Aspergillus flavo furcatis: Aflatoxin test and milk-clotting protease production in submerged and solid state fermentation
February 2017
Proteolytic enzymes are metabolites that can be produced by microbial sources and develop important functions in food industry as in cheese manufacturing. However, it is necessary to ensure the final product safety by testing toxins production by microorganisms. As a result of this, the aim of this study was to investigate the production of proteases by Aspergillus flavo furcatis DPUA 1608 in submerged and solid state...
Use of novel specific primers targeted to pheS and tuf gene for species and subspecies identification and differentiation of the Bacillus subtilis subsp. subtilis, Bacillus amyloliquefaciens and Bacillus licheniformis
February 2017
To clearly delimit the members of the Bacillus subtilis group (BSG), is difficult using common phenotypic and genotypic methods. This study described the use of pheS and tuf gene as targets for interspecies discrimination within the BSG, and also to develop specific PCR and SNP primers for species and subspecies identification and differentiation. The average sequence similarity values of the pheS and tuf gene among...
Multidrug resistant Campylobacter in faecal and carcasses of commercially produced poultry
February 2017
Poultry meat and products are major transmission routes of human campylobacteriosis. The aim of this study was to determine the numbers and antibiogram profile of Campylobacter isolates from slaughtered broiler and layer birds. One hundred and sixty caecal and one hundred and thirty two carcasses were randomly sampled at the Kejetia poultry slaughter, isolated on charcoal-cefoperazone-deoxycholate agar (CCDA) and...
Co-existence of multiple B-lactamase traits among clinical isolates of Escherichia coli from rural part of Maharashtra, India
February 2017
Acquisition of multidrug resistance and the spread of MDR among pathogenic microorganisms is a growing concern to world. Extended-spectrum ï¢-lactamases (ESBLs) strains of the Enterobacteriaceae; ESBL trait expressing Escherichia coli poses major threat and ESBL E. coli infections are increasingly reported from hospital settings as well as community settings with rising mortality and morbidity rates. The objective of...
Use of a combined cultural-molecular method for isolation and identification of Campylobacter from broiler chicken in Morocco
February 2017
Campylobacters are very important foodborne pathogens that raise the interest of food processors, researchers, as well as consumers and all stakeholders. Their contaminations can result in life threatening disorders, potentially leading to chronic sequelae such as Reiter’s and Guillain-Barré syndromes or Crohn’s Disease. Poultry has been identified as the main and most common reservoirs for...
Post-treatment of cachaca (Brazilian sugarcane spirit) with charcoal made from cane bagasse
February 2017
The objective of this study was to ferment sugarcane juice using two types of yeast and evaluate the quality of the distillate after beverage filtration in a column filled with ground charcoal derived from cane bagasse. The experimental design was randomized block (split plots, 2 x 2) with three replicates. The primary treatment involved two types of yeast: CA-11 and baker’s yeast. Secondary treatment involved...
Prevalence of IgG and IgM antibodies to Chikungunya virus among outpatients with febrile illness attending University of Maiduguri Teaching Hospital, Maiduguri, Borno State, Nigeria
February 2017
In Nigeria, there is paucity of information on the epidemiology of infections due to Chikungunya virus (CHIKV) especially among patients with febrile illness. Cases of febrile illness are usually associated with malaria and typhoid fever without considering the possibility of viral aetiology. This study was designed to determine the prevalence and identify other epidemiological parameters of CHIKV infections among...
In vitro antifungal effect of potassium sorbate and sodium benzoate on the growth of fungi causing sinusitis
February 2017
The study involved 70 nasal swab collected from people suffering from sinusitis and consulted the Ear, Nose and Throat Unit in Al-Diwaniya Teaching Hospital, Iraq during the period from 12 June to 12 August 2016 with ages ranging from 0-65 years old. The results showed that the number of nasal swabs that gave fungal growth was 36/70 (51.428%); highest percentage of fungal infection was recorded in ages ranging from...
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