African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6688

Full Length Research Paper

Regeneration of cotyledonary nodes from the recalcitrant melon cultivar ‘Gaúcho’

Marcelo Nogueira do Amaral
  • Marcelo Nogueira do Amaral
  • Department of Botany, Institute of Biology, Federal University of Pelotas (UFPel), RS, Brazil.
  • Google Scholar
Fabiana Roos Nora
  • Fabiana Roos Nora
  • Center for Biotechnology, Technology Development Center, Federal University of Pelotas (UFPel), RS, Brazil.
  • Google Scholar
Luciano da Silva Pinto
  • Luciano da Silva Pinto
  • Center for Biotechnology, Technology Development Center, Federal University of Pelotas (UFPel), RS, Brazil.
  • Google Scholar
Juliana Aparecida Fernando
  • Juliana Aparecida Fernando
  • Department of Botany, Institute of Biology, Federal University of Pelotas (UFPel), RS, Brazil.
  • Google Scholar
Jose Antonio Peters
  • Jose Antonio Peters
  • Department of Botany, Institute of Biology, Federal University of Pelotas (UFPel), RS, Brazil.
  • Google Scholar


  •  Received: 04 February 2014
  •  Accepted: 07 April 2014
  •  Published: 12 August 2014

Abstract

There is lack of suitable explants for efficient shoot proliferation in recalcitrant melon cultivars restricts genetic manipulation for crop improvement. In this study, we aimed to stimulate the regeneration capability of cells of melon (Cucumis melo L.) explants in order to increase shoot proliferation efficiency during regeneration. The media of seeds germination containing different concentrations of benzylaminopurine (BAP: 0.5, 1.0, and 1.5 mg L-1) can stimulate the shoot proliferation potential of cotyledonary nodes was described. The highest number of shoots per explant was obtained when the seeds and cotyledonary nodes were cultured on Murashige and Skoog media containing 1.0 and 0.5 mg L-1 BAP, respectively, and the regeneration process occurred through organogenesis.

 

Key words: Cucumis melo L., organogenesis, cytokinin, explants.