African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6688

Full Length Research Paper

Acaricide effect of some extracts and fractions on Tetranychus urticae Koch (Acari: Tetranychidae)

Mustapha Elkertati1,2,3*, Abdelali Blenzar1, Abdelmalek Boutaleb Jotei2, Ilham Belkoura3 and Bouchra Tazi3
1Department of Biology, Faculty of Sciences, Moulay Ismail University, BP 11201 Zitoune, Meknès, Morocco. 2Department of Plants and Environment Protection, National School of Agriculture, km 10, Route Haj Kaddour, B.P. S/40, Meknès, Morocco. 3Department of Basic Sciences, National School of Agriculture, km 10, Route Haj Kaddour, B.P. S/40, Meknès, Morocco.
Email: [email protected]

  •  Accepted: 13 June 2013
  •  Published: 20 June 2013


The acaricidal potential of Melia azedarachPeganum harmalaNigella sativa and Trigonella foenum-graecumseeds was investigated on adult Tetranychus urticae under laboratory conditions. After the treatment of foliar discs using dichloromethane and ethanol extracts of these seeds, the statistical analysis of the mortality percentage revealed that N. sativa was the most potent one. In fact, using the dichloromethane extracts, the LC50s were 398.64, 410.48, 73.95 and 520.74 ppm for the four species respectively. Whereas, for ethanol extracts, the LC50s were 994.93, 617.26, 92.96 and 1074.81 ppm respectively. n-Hexane, trichloromethane and ethyl acetate fractions of N. sativa dichloromethane extract used in the same manner showed that n-Hexane fraction was the most toxic with LC50=74.34 ppm followed by trichloromethane fraction with 96.74 ppm then ethyl acetate with 104.27 ppm. The phytochemical analysis of these fractions revealed the presence of some metabolites which could explain their toxicity.


Key words: Acaricidal potentials, extract, fractions, ethanol, dichloromethane, Tetranychus urticae, phytochemical analysis