The aim of this study was to determine the bacterial causes, their antibiogram and prevalence of intramammary infection (IMI) in the buffaloes and quarters of Azeri ecotype in Tabriz, Iran and assess any relationships between IMI and somatic cell count (SCC). After conducting California Mastitis Test (CMT) in farms, quarter- based milk samples were collected from 300 buffaloes. Also 150 samples were collected for bacterial culture and count. Based on CMT, 13.87% of quarters and 23.66% of buffaloes had subclinical mastitis, the sensitivity and specificity of CMT for infections with all bacteria and infections with major pathogens were 55 and 67.39, 70 and 50% respectively. Coagulase-negative staphylococci (CNSs) were the most common pathogens. Isolated bacteria had no significant effects on mean SCC of infected quarters. The most effective antibiotics against all isolated agents were Cephtiofur and Trimetoprim+ Sulfametoxazol with a sensitivity rate of 97.3and 94.6% respectively. The SCC and total bacterial count (TBC) of infected quarters were significantly higher than healthy ones (p< 0.05). Based on the results of current study, CMT has acceptable sensitivity and specificity in diagnosis. Coliforms are not probably very important in buffaloes intramammary infections.
Key words: Buffalo, mastitis, intramammary infection, SCC, bacterial culture.
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