African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6691

Full Length Research Paper

Effects of low nitrogen on chlorophyll content and dry matter accumulation in maiz

S. Sen
  • S. Sen
  • Department of Plant Breeding and Genetics, Cornell University, 14853 Ithaca, USA.
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M. E. Smith
  • M. E. Smith
  • Department of Plant Breeding and Genetics, Cornell University, 14853 Ithaca, USA.
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T. Setter
  • T. Setter
  • Department of Plant Breeding and Genetics, Cornell University, 14853 Ithaca, USA.
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  •  Received: 02 March 2015
  •  Accepted: 18 September 2015
  •  Published: 24 March 2016

Abstract

For photosynthesizing plants, studies on the effect of different levels of Nitrogen on chlorophyll could let us know about the overall physiological status of the plant under different environmental conditions. Objective of our study was to understand if there was any difference among the ten recombinant inbred lines (RILs) of IBM population and their parents B73 and Mo17 for chlorophyll content and dry matter accumulation in their response to the N stress. Five IBM genotypes that carry predominantly B73 alleles and five IBM genotypes that carry Mo17 alleles at 5 quantitative trait loci (QTL) regions associated with root traits and N use efficiency from the published Maize Genome Database were evaluated in this study along with their parents. Plants were grown in the Guterman Green House (Cornell University, USA). Two nitrogen treatments (solution with high N contained 65.79 g Ca (NO3)2 4H2O in 100L of 1X solution making 5.0mM NO3 and solution with low N contained 2.63 g Ca (NO3)2 4H2O in 100L of 1X solution making 0.2 mM NO3) were given. Plant traits like leaf dry weight, stalk dry weight and root dry weight were observed. Chlorophyll content was estimated to measure the effect of different N levels on photosynthetic activity.  Genotype with high in B73 composition had relative advantage over Mo17 in chlorophyll content, and dry weight of roots under low N condition. The highest root-shoot ratio under low and high N was observed in IBM056 and IBM153 respectively. Except for IBM153 and IBM337, all other genotypes showed reduced LFS at high N. The result showing lower root-shoot ratio and leaf fraction of shoot (LFS) under the high N treatment suggests that shoot growth increases more than root growth in response to increased N application and that within the shoot, stem growth increases more than leaf growth. Results support the conclusion that under low N condition, shoot growth is retarded than root growth.

 

Keywords: Nitrogen, Maize, Chlorophyll, Dry matter accumulation, Photosynthesis.