African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6691

Full Length Research Paper

Trypsin (serine protease) inhibitors in peanut genotypes aiming for control of stored grain pests

Patrícia de Lima Martins
  • Patrícia de Lima Martins
  • Universidade Estadual da Paraíba – UEPB, Pós-graduação em Ciências Agrárias, Rua Baraúnas n. 351, CEP: 58429-500 Bairro Universitário, Campina Grande, PB, Brazil.
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Roseane Cavalcanti dos Santos
  • Roseane Cavalcanti dos Santos
  • Empresa Brasileira de Pesquisa Agropecuária, Embrapa Algodão. Rua Oswaldo Cruz n. 1143, CEP: 58428-095, Centenário, Campina Grande, PB, Brazil.
  • Google Scholar
João Luis da Silva Filho
  • João Luis da Silva Filho
  • Empresa Brasileira de Pesquisa Agropecuária, Embrapa Algodão. Rua Oswaldo Cruz n. 1143, CEP: 58428-095, Centenário, Campina Grande, PB, Brazil.
  • Google Scholar
Fábia Suelly Lima Pinto
  • Fábia Suelly Lima Pinto
  • Empresa Brasileira de Pesquisa Agropecuária, Embrapa Algodão. Rua Oswaldo Cruz n. 1143, CEP: 58428-095, Centenário, Campina Grande, PB, Brazil.
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Liziane Maria de Lima
  • Liziane Maria de Lima
  • Empresa Brasileira de Pesquisa Agropecuária, Embrapa Algodão. Rua Oswaldo Cruz n. 1143, CEP: 58428-095, Centenário, Campina Grande, PB, Brazil.
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  •  Received: 20 May 2014
  •  Accepted: 26 June 2014
  •  Published: 17 July 2014

Abstract

Peanut seeds from different genotypes were evaluated for activities of trypsin inhibitors (serine protease), based on in vitro and in vivo assays, aiming to further selection of genitors in breeding programs for tolerance of stored grain pests. The in vitro assays were based on inhibition of insect digestive enzymes and also on thermal and pH stabilities of seed protein, while the in vivo assays were performed with insects Alphitobius diaperinus, Tribolium castaneum, Tenebrio molitor and Spodoptera frugiperda. Seed inhibitors of all genotypes inhibited bovine trypsin at 70 to 94%. The seed extract of BRS Havana inhibited T. castaneum and T. molitor up to 80% while the extract of BRS 151 L7 inhibited A. diapennus at nearly 20%. The seed inhibitors of both cultivars were stable at 80°C and also at different pH values. The two peanut genotypes are recommended as promising parents for breeding program aiming to selecting lines with tolerance to Tenebrio and Alphitobius insects.

 

Key words: Trypsin inhibitor, Arachis hypogaea, lepidoptera, coleoptera.