African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6688

Full Length Research Paper

Biocontrol of Phytophthora parasitica and Fusarium oxysporum by Trichoderma spp. in Hibiscus sabdariffa plants under field and greenhouse conditions

Eduardo Osorio-Hernández
  • Eduardo Osorio-Hernández
  • Colegio de Postgraduados. 56230, Montecillo, Estado de México.
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Javier Hernández-Morales
  • Javier Hernández-Morales
  • Colegio de Postgraduados. 56230, Montecillo, Estado de México.
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Víctor Conde-Martínez
  • Víctor Conde-Martínez
  • Colegio de Postgraduados. 56230, Montecillo, Estado de México.
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Alejandro Casimiro Michel-Aceves
  • Alejandro Casimiro Michel-Aceves
  • Centro de Estudios Profesionales del Colegio Superior Agropecuario del Estado de Guerrero, Av. Vicente Guerrero No. 81, C. P. 40000. Colonia Centro. Iguala, Guerrero, México.
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Juan Cibrián-Tovar
  • Juan Cibrián-Tovar
  • Colegio de Postgraduados. 56230, Montecillo, Estado de México.
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Humberto Vaquera-Huerta
  • Humberto Vaquera-Huerta
  • Colegio de Postgraduados. 56230, Montecillo, Estado de México.
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  •  Received: 01 January 2014
  •  Accepted: 16 April 2014
  •  Published: 05 May 2014

Abstract

The efficiency of the biological control agent Trichoderma spp was tested against Phytophthora parasitica and Fusarium oxysporum in Roselle plants under field and greenhouse conditions. Under field conditions, three Trichoderma doses were applied to the base of the plant, and azoxystrobin and benomyl were applied at 30 and 60 days after planting. Also, 30 day-old plants were inoculated with 7 g of corncob/Trichoderma under greenhouse conditions, and inoculated with F. oxysporum and P. parasitica 3 or 4 days later. Field results showed that Trichoderma longibrachiatum-treated plants had 15% less incidence of black shank, and in one of the experiments, calyx dry weight was higher. Under greenhouse conditions, plants inoculated with Trichoderma gamsii and T. longibrachiatum had between 20 and 30% less incidence of F. oxysporum and P. parasitica, respectively. Both Trichoderma species increased fresh and dry plant weight, and T. gamsii also increased plant height. In general, T. gamsii and Trichoderma virens increased height, fresh and dry weight of Roselle plants infected with F. oxysporum.

 

Key words: Trichoderma virens, Trichoderma longibrachiatum, Trichoderma asperellum, Trichoderma gamsii, Roselle.