African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6691

Full Length Research Paper

Plant defense elicitors’ purification in soybean and bean from pathogenic nematode

Edilaine Della Valentina Gonçalves-Trevisoli*
  • Edilaine Della Valentina Gonçalves-Trevisoli*
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon – Paraná – Brazil, street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Center, Brazil.
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Jose Renato Stangarlin
  • Jose Renato Stangarlin
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon – Paraná – Brazil, street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Center, Brazil.
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Bruna Broti Rissato
  • Bruna Broti Rissato
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon – Paraná – Brazil, street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Center, Brazil.
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Omari Dangelo Forlin Dildey
  • Omari Dangelo Forlin Dildey
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon – Paraná – Brazil, street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Center, Brazil.
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Sidiane Coltro-Roncato
  • Sidiane Coltro-Roncato
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon – Paraná – Brazil, street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Center, Brazil.
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Laline Broetto
  • Laline Broetto
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon – Paraná – Brazil, street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Center, Brazil.
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Heloísa Ferro Constancio Mendonça-Müller
  • Heloísa Ferro Constancio Mendonça-Müller
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon – Paraná – Brazil, street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Center, Brazil.
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Janaína Dartora
  • Janaína Dartora
  • Instituto Agronômico do Paraná/Área de Produção e Experimentação, Pato Branco – Paraná - Brazil, highway BR-158, 5.517 SR - Bom Retiro, Zip Code 85501-970, Brazil.
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Cristiane Claudia Meinerz
  • Cristiane Claudia Meinerz
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon – Paraná – Brazil, street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Center, Brazil.
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Luciana Iurkiv
  • Luciana Iurkiv
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon – Paraná – Brazil, street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Center, Brazil.
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Thaisa Muriel Mioranza
  • Thaisa Muriel Mioranza
  • State University of West Maringá – UEM, Maringá– Paraná – Brazil, street Colombo, Number 5790, Zip Code: 87020-900, Center, Brazil.
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Daliana Hisako Uemura-Lima
  • Daliana Hisako Uemura-Lima
  • State University of West Paraná, Marechal Cândido Rondon – Paraná – Brazil, street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Center, Brazil.
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Tulya Fernanda Barrientos Webler
  • Tulya Fernanda Barrientos Webler
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon – Paraná – Brazil, street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Center, Brazil.
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Odair Jose Kuhn
  • Odair Jose Kuhn
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon – Paraná – Brazil, street Pernambuco, Number 1777, Zip Code: 85960-000, Box: 91, Center, Brazil.
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  •  Received: 03 August 2016
  •  Accepted: 31 October 2016
  •  Published: 08 December 2016

Abstract

Plant resistance induction against pathogens is an alternative disease control method, which involves the activation of the plant defense mechanisms, such as the phytoalexins induction. The eliciting molecules have the capacity of inducing and activating such responses and thus, techniques have searched to isolate and characterize fractions with eliciting aspect. This study aimed to purify, through ion exchange chromatography and gel filtration chromatography, eliciting molecules from phytopathogenic nematodes, and test them in the induction of phaseolin in bean hypocotyls and gliceolin in soybeans cotyledons. A Tris HCl 0.05 M (pH 6.8) buffer was used as control treatment and acibenzolar-S-methly (50 mg i.a. L-1) and Saccharomyces cerevisiae (20 mg m L-1) was used as induction standard treatment. Ion exchange chromatography (IEC) and gel filtration chromatography (GFC) were applied to separate power eliciting fractions from five hundred female root knot nematodes (Meloidogyne javanica). The purification of elicitors, through IEC, resulted in sixty glicidic fractions and six glycoprotein ones. They were classified according to their nature, being twenty-six glicidic fractions and thirty-seven glycoprotein ones, with molecular masses ranging from 29.19 to 2,989.25 kDa. From the purified fractions, eight of them presented phaseolin inducing effect potential, whereas fifteen fractions presented gliceolin inducing effect. Chromatography proved to be efficient in purifying the eliciting compounds. Compounds having gliceolin and phaseolin suppressing characteristics were verified in the bioassays. For those fractions obtained through IEC and posteriorly submitted to GFC that did not induce phytoalexin, it is suggested that the molecules need to act jointly so there is eliciting effect and thus induce a defense response in the plant.

Key words: gel filtration chromatography, ion exchange chromatography, Meloidogyne javanica, phytoalexins, resistance induction.