African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6691

Full Length Research Paper

Medium-term conservation and regrowth of the tropical multipurpose species Genipa americana

Ana da Silva Ledo
  • Ana da Silva Ledo
  • Embrapa Coastal Tablelands, Avenida Beira Mar 3250, Aracaju, Sergipe, Brazil.
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Camila Santos Almeida
  • Camila Santos Almeida
  • Federal University of Sergipe, Sao Cristovao, Sergipe, Brazil.
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Ana Veruska Cruz da Silva
  • Ana Veruska Cruz da Silva
  • Embrapa Coastal Tablelands, Avenida Beira Mar 3250, Aracaju, Sergipe, Brazil.
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Josue Francisco da Silva Junior
  • Josue Francisco da Silva Junior
  • Embrapa Coastal Tablelands, Avenida Beira Mar 3250, Aracaju, Sergipe, Brazil.
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Aparecida Gomes de Araujo
  • Aparecida Gomes de Araujo
  • Embrapa Coastal Tablelands, Avenida Beira Mar 3250, Aracaju, Sergipe, Brazil.
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  •  Received: 11 July 2014
  •  Accepted: 21 August 2015
  •  Published: 08 October 2015

Abstract

The Genipa americana L., a fruit-producing species found in several countries and commonly found in the Brazilian Northeast, is very important economically, both for its wood and as a source of food and dye. This study evaluate the effects of abscisic acid (ABA) and the concentrations of salt and sugar in MS medium on reducing Genipa americana L. growth for in vitro conservation, as well as the effects of benzylaminopurine (BAP) on the regrowth phase. Seedlings from accession CZA, after 90 days of in vitro cultivation, were transferred to Murashige and Skoog (MS) medium with 30 g L-1 sucrose and various concentrations of ABA (0, 0.5, 1.0, 2.0 and 4.0 mg L-1). In a second assay, various concentrations of MS medium salts and sucrose were tested. During the regrowth phase, nodal segments were placed in MS regeneration medium with 30 g L-1 sucrose and BAP (0 or 1 mg L-1). All the ABA concentrations affected the number of leaves, but significant effects on the length of aerial portion were not observed. The MS and ½ MS media with 30 g L-1 sucrose slowed plant growth and are recommended for conservation protocols for slow growth. Adding 1 mg L-1 BAP to the culture medium caused a greater morphogenic response, and the apical, medial and basal nodes retained morphogenic potential during regrowth.

 

Key words: Genipa americana L., germplasm, abscisic acid, sucrose.