African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6691

Full Length Research Paper

In vitro regeneration of Treculia africana Decne. from embryo explants on different nutrients and sucrose conditions

Isaac, U. G.
  • Isaac, U. G.
  • Plant Biotechnology Research Laboratory, Department of Plant Science and Biotechnology, Faculty of Biological Sciences, University of Nigeria Nsukka, Enugu State, Nigeria.
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Okafor, C. U.
  • Okafor, C. U.
  • Plant Biotechnology Research Laboratory, Department of Plant Science and Biotechnology, Faculty of Biological Sciences, University of Nigeria Nsukka, Enugu State, Nigeria.
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Okezie, C. E. A
  • Okezie, C. E. A
  • Plant Biotechnology Research Laboratory, Department of Plant Science and Biotechnology, Faculty of Biological Sciences, University of Nigeria Nsukka, Enugu State, Nigeria.
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  •  Received: 05 March 2018
  •  Accepted: 24 April 2018
  •  Published: 31 May 2018

Abstract

The study is essential in reducing germination time of Treculia africana var. inversa. The effect of three different concentrations of sucrose namely 2, 3 and 4% were investigated on the in vitro regeneration of embryo explants of T. africana Decne. on the media of Murashige and Skoog (MS) and Gamborg et al. (B5)  respectively without any growth regulator. The experimental design was a 2 × 5 factorial in a completely randomized design with each treatment consisting of ten replicates. Results showed that while both media including control (contains agar only) supported the in vitro regeneration of T. africana embryo explants, B5 medium was found to be significantly superior (P≤0.05) to MS medium in all the growth parameters studied. B5 medium at 4% sucrose elicited the best response in all the growth parameters determined while control gave the least response. The protocol reported here can be used for large scale propagation of true-to-type T. africana plants within a short time for the purpose of improvement through genetic transformation (mutagenesis) and the development of a viable conservation programme.
 
Key words: Treculia africana, Murashige and Skoog (MS) medium and Gamborg et al. (B5) medium, embryo explant.