African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6900

Full Length Research Paper

Molecular characterization of some brinjal genotypes (Solanum melongena L) using simple sequence repeat (SSR) markers

Farid Ahmed
  • Farid Ahmed
  • Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh.
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Md. Golam Rabbani
  • Md. Golam Rabbani
  • Department of Horticulture, Bangladesh Agricultural University, Mymensingh, Bangladesh.
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Md. Rafiqul Islam
  • Md. Rafiqul Islam
  • Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh.
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Mostafezur Rahman
  • Mostafezur Rahman
  • Department of Horticulture, Bangladesh Agricultural University, Mymensingh, Bangladesh.
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Md. Abdul Malek
  • Md. Abdul Malek
  • Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh.
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Mirza Mofazzal Islam
  • Mirza Mofazzal Islam
  • Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh.
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Reza Mohammad Emon
  • Reza Mohammad Emon
  • Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh.
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  •  Received: 05 September 2019
  •  Accepted: 21 November 2019
  •  Published: 31 December 2019

Abstract

To analyze genetic variability of 48 brinjal genotypes by Simple Sequence Repeat (SSR) markers, four primers named smSSR01, smSSR03, smSSR11 and smSSR14 were selected for analyze the data. Four primers generated clear bands and a total of 44 alleles were detected among the 48 brinjal genotypes (9.0 to 14.0 alleles per locus with a mean of 11.0 alleles per locus). According to Nei’s (1973), the highest level of gene diversity value 0.8672 was observed in loci smSSR11 and the lowest level of gene diversity value 0.6580 was observed in the loci smSSR14 with a mean diversity of 0.7847. As a measure of the informativeness of microsatellites, the PIC value ranges from a low of 0.6413 (smSSR14) to a high of 0.8536 (smSSR11) and averaged 0.7660. The genetic distance (GD) between genotypes was computed from combined data for the four primers, ranging from 0.250 to 1.000. The highest genetic distance (1.000) was observed between Morich Begun (Small) vs. Laffa (Long, Violet), Morich Begun (Small) vs. BARI Begun -1 and many other genotype pairs and also the lowest genetic distance (0.250) was found in Morich Begun (Small) vs. Pahoza-2, Morich Begun (Small) vs. BAU Begun-1, Singnath (Long, Violet) vs. Laffa BAU (Long, Violet), Borka (Long, Green) vs. Purple Long and many other genotype pairs. The Unweighed Pair Group Method with Arithmetic Means (UPGMA) cluster tree analysis lead to the grouping of the 48 genotypes into seven major clusters and the genotypes that are derivatives of genetically similar type form cluster together. Maximum brinjal genotypes include cluster IV. The SSR markers used in the present study were able to differentiate forty eight brinjal genotypes genetically. The results of the study suggested that SSR can be used as a suitable genetic marker to identify the brinjal genotypes. However, further studies involving large number of genotypes and primers need to be conducted to get more precise information and help enhance the knowledge of students and researchers relating to molecular characterization of other solanaceious crops.

Key words: Brinjal, SSR, Genetic Diversity

 

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This article is published under the terms of the Creative Commons Attribution License 4.0

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