African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6692

Full Length Research Paper

Biochemical and molecular investigations on prime mechanisms of induced resistance in French bean (Phaseolus vulgaris L.) and soybean (Glycine max L.)

Haddad El Rabey1,2*, Suzan Abdallah3 and Attia Ahmed3
  1Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia. 2Genetic Engineering and Biotechnology Institute, Minufiya University, Sadat City, Egypt. 3Faculty of Science, Benha University, Benha, Egypt.
Email: [email protected]

  •  Accepted: 11 October 2011
  •  Published: 26 November 2011

Abstract

 

The prime mechanisms of induced resistance in French bean and soybean plants were investigated against three pathogenic fungi, namely Fusarium solani f. sp. phaseoli, Fusarium solani f. sp. glycine andColletotricum lindemothianum. This latter fungal pathogen was chosen for a comparative purpose, as it represents a non related genus of the main fungal pathogens used in this study. The efficacy of the induced resistance was tested using four different incubation temperatures (20, 25, 30 and 35°C), in combination, with the use of the non pathogenic Fusarium, isolated from potatoes, served as an inducer or elicitor for 4 h and, subsequently, the challenged hypocotyls were inoculated with the pathogenic isolate of F. solani f. sp.phaseoli in all the pathosystems tested in this study. Among the tested cultivars, Nebraska was moderately resistant and Crowford was moderately susceptible to Fusarium infection, whereas the other tested cultivars were susceptible. randomly amplified polymorphic DNA (RAPD) fingerprinting profiles characterized the tested cultivars and pathogens genomes, whereas SDS protein electrophoresis showed the newly induced proteins as a result of prime mechanisms of induced resistance.

 

Key words: French bean, prime resistance, soybean, Fusarium, randomly amplified polymorphic DNA (RAPD).