Okra is an economically important vegetable crop cultivated in tropical and sub-tropical parts of the world with a multipurpose due to its various uses of the fresh leaves, buds, flowers, pods, stems and seeds. Thus, characterization and quantification of the genetic diversity and information on the genetic diversity within and among closely related crop genotypes is essential for a rational use of plant genetic resources in crop improvement program. In this context, a field experiment was conducted during 2020/21 cropping season at Abrahamo testing site of Assosa zone in north western Ethiopia with objective to estimate the variability of okra accessions with respect to important qualitative and quantitative traits. Treatments consisted in 19 okra accessions (AB 29411, AB 29615, AB 40416, AB 29415, AB 240613, AB 294009, AB 29052, AB 29417, AB 29407, AB 29410, AB 29413, AB 240584, AB 29616, AB 29414, AB 29412, AB 29408, AB 29051, AB 24612 and AB 240589)which were obtained from Ethiopian Biodiversity Institute (EBI). The treatments were laid out in a randomized complete block design (RCBD) with three replications. All the qualitative characteristics recorded for genotypes showed different modalities and frequencies at vegetative stage with 85% of genotypes with erect growth type, 95% with green stem colour, 57% with large leaf blade size, 58% with small flower, 51.4% with convex shape surface veins, 37.2% with deep lobbing, 64.8% strong dentation of margins, 63% with conspicuous leaf pubescence and 64.5% with prickly fruit pubescence. With respect to quantitative traits, genotypes AB 29410, AB 29417, AB29411, AB 240584 and AB 29413 gave relatively higher fresh fruit yield (> 15,000 kg/ha) with the highest fresh fruit yield was recorded for genotype AB 29410 Agronomic and morphological evaluations have provided more accurate estimation of genetic diversity, the raw material of plant breeding. In general this study enabled to determine how the influential traits direct to improve bambara groundnut genotypes

Keywords: Crop, Fruit yield, Genotypes, , Variability , Heritability, Vegetable