African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12500

Review

Advances in highly specific plant gene silencing by artificial miRNAs

Guangyu Luo
  • Guangyu Luo
  • Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, Hunan Province, 410128, China.
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Ting Su,
  • Ting Su,
  • Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, Hunan Province, 410128, China.
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Cuifang Liu
  • Cuifang Liu
  • Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, Hunan Province, 410128, China.
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Jie Zou
  • Jie Zou
  • Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, Hunan Province, 410128, China.
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Ailing Liu
  • Ailing Liu
  • Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, Hunan Province, 410128, China.
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Xinbo Chen*
  • Xinbo Chen*
  • Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, Hunan Province, 410128, China.
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  •  Received: 10 November 2010
  •  Accepted: 18 June 2013
  •  Published: 07 May 2014

Abstract

Endogenous microRNAs (miRNAs) are potent negative regulators of gene expression in plants and animals. Through artificially transformed miRNA (amiRNAs) to target one or several genes of interest is becoming a powerful tool for silencing genes. The characteristics and application prospect of artificial microRNA (amiRNA) technology were reviewed.

 

Key words: Gene-silence, RNA interference, MiRNA, Artificial-microRNA.

Abbreviation

Abbreviations: amiRNA, Artificial microRNA; RNAi, RNA interference; sRNAs, small silencing RNAs; hpRNAi, hairpin RNA interference; premiRNA, miRNA precursors; RdRP, RNA-dependent RNA polymerase.

Copyright © 2025 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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