African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12500

Full Length Research Paper

Characterization of Pseudomonas aeruginosa PB112 (JN996498) isolated from infected Labeo bata (Hamilton) by 16S rRNA gene sequence analysis and fatty acid methyl ester (FAME) analysis

  Somerita Panda1*, P. K. Bandyopadhyay1 and S. N. Chatterjee 2        
  1Parasitology Laboratory, Department of Zoology, University of Kalyani, Kalyani, West Bengal, India. 2Parasitology and Microbiology Laboratory, University of Burdwan, Burdwan, West Bengal, India.
Email: [email protected], [email protected]

  •  Accepted: 24 October 2012
  •  Published: 23 January 2013

Abstract

 

In this study, a total number of fifteen moribund Labeo bata were collected from Hooghly, Purba Medinipur, and South 24 Parganas districts of West Bengal, India. The lesions and hemorrhages were recorded at the base of the fins or on the skin due to bacterial infection. Isolation and pure culture of the causative agent were made in the laboratory. The 16S rRNA gene sequence of the bacterial isolate PB112 has been deposited in the NCBI GenBank and was assigned the accession number JN996498. The GC and AT contents of the 16S rRNA gene sequence (JN996498) were 54.22 and 45.78%, respectively. Based on the biochemical properties, 16S rRNA gene sequence analysis and fatty acid methyl ester (FAME) analysis, the causative bacteria were identified as Pseudomonas aeruginosa.

 

Key words: Labeo bata, Pseudomonas sp., 16S rRNA gene sequence, fatty acid methyl ester (FAME) analysis.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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