African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12502

Full Length Research Paper

Optimization of growth and extracellular glucoamylase production by Candida famata isolate

Lagzouli Mohamed1*, Mennane Zakaria1, Aitounejjar Ali2, Wafae Senhaji3, Ouhssine Mohamed1, Elyachioui Mohamed1, Berny EL Hassan1 and Jadal Mohamed
1Laboratory of Microbial Biotechnology, University IBN TOFAIL, Department of Sciences, BP 133, 14000 Kenitra-Morocco. 2National Institute of Agronomic Researches, INRA, BP: 589 Settat 26000, Morocco. 3Laboratoire de Recherche et d'Analyses Médicales de la Gendarmerie Royale, avenue Ibn Sina-Agdal-Rabat, B.P 8087, 10000, Morocco.
Email: [email protected]

  •  Accepted: 16 March 2007
  •  Published: 19 November 2007

Abstract

Candida famata was isolated from traditional Moroccan sourdough. It exhibited high glucoamylase and biomass production. Starch induces high glucoamylase production C. famata with maximum glucoamylase activity at 5 g/L. Glucose stimulates good production in biomass but strongly inhibits glucoamylase production. Among the sources of nitrogen tested, yeast extract and the (NH4)2HPO4 gave maximum glucoamylase and biomass after 72 h of incubation in liquid medium at 30°C, pH 5 and 105 rpm.

 

Key words: Glucoamylase, yeast, optimization, biotechnology, Candida famata.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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