African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12412

Full Length Research Paper

Prevalence of drug resistance and genetic characterization of Mycobacterium tuberculosis complex strains from pulmonary tuberculosis patients co-infected with malaria at Jamot Hospital in Yaoundé

Tiani Lionel Ulrich
  • Tiani Lionel Ulrich
  • Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon.
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Ambassa Axel Cyriaque
  • Ambassa Axel Cyriaque
  • Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon.
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Assam Jean Paul Assam
  • Assam Jean Paul Assam
  • Laboratory for Tuberculosis Research and Pharmacology, Biotechnology Center, University of Yaoundé 1, Cameroon.
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Andoseh Genevieve
  • Andoseh Genevieve
  • Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon.
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Zeukeng Francis
  • Zeukeng Francis
  • Faculty of Sciences, University of Buea, Cameroun.
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Tchinda Cedric Fossi
  • Tchinda Cedric Fossi
  • Laboratory for Tuberculosis Research and Pharmacology, Biotechnology Center, University of Yaoundé 1, Cameroon.
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Tsasse Martine Augusta Flore
  • Tsasse Martine Augusta Flore
  • Laboratory for Tuberculosis Research and Pharmacology, Biotechnology Center, University of Yaoundé 1, Cameroon.
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Nkah Numfor Leonard
  • Nkah Numfor Leonard
  • Center for research on Medecinal plants and Traditional Medecine, Institute of Medical Research and medicinal plants studies (IMPM), Ministry of Scientific Research and innovation,Yaoundé, Cameroon.
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Gonsu Kamga Hortense
  • Gonsu Kamga Hortense
  • Cantam Project Manager/chef de projet, Fondation Congolaise pour la Recherche Médicale, Campus WHO/AFRO, Villa D6, Brazzaville, Rep Congo.
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Kouanfack Charles
  • Kouanfack Charles
  • Department of Microbiology and Infectious Diseases, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Cameroon.
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Pefura Yone Eric Walter
  • Pefura Yone Eric Walter
  • Hôpital du Jour, Central Hospital in Yaoundé, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon.
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Djuidje Ngounoue Marceline
  • Djuidje Ngounoue Marceline
  • Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon.
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Bigoga Jude Daiga
  • Bigoga Jude Daiga
  • Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon.
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Ntoumi Francine
  • Ntoumi Francine
  • Ethics Committee on Health Research in Central Africa (CERSAC), Cameroon.
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Beng Véronique Penlap
  • Beng Véronique Penlap
  • Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon.
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  •  Received: 04 July 2022
  •  Accepted: 26 August 2022
  •  Published: 30 September 2022

Abstract

Tuberculosis (TB) is caused by the Mycobacterium tuberculosis complex (MTBC) and remains a major global public health concern. This study aimed to determine the prevalence of drug resistance and the genetic variation among MTBC population in pulmonary tuberculosis patients co-infected with Malaria at Jamot Hospital in Yaoundé-Cameroon. This was a 12 months cross-sectional study that enrolled 336 participants aged 15 years and above. Following sputum culture on solid media, drug resistance was detected using the proportion method and later confirmed by the Line Probe Assay. Isolates were further subjected to molecular characterization using spoligotyping. Amongst the 336 TB patients registered in this study, there were 17 (5.05%) TB-Malaria co-infected patients. Overall, in 25 (12.88%) patients the bacteria were resistant to at least one anti-TB drug, of which, 3 (1.54%) were co-infected with malaria. Multidrug-resistance (MDR) was observed in 2 cases (1.02%), 1 (0.51%) of which was in a TB-Malaria co-infected patient. M. tuberculosis was the only species identified. The T1 (60%) and the LAM10_CAM (27.5%) families were the most prevalent genetic families both in TB-malaria co-infected and in mono-infected TB patients. The description of drug resistance prevalence and of the M. tuberculosis genetic diversity is expected to contribute to improving the TB case management in Cameroon.

 

Key words: Pulmonary tuberculosis, multidrug resistance, line probe assay, spoligotyping, genetic diversity, HJY, co-infection.