Abstract

Cadmium and mercury present in the environment, cause blood disorders. This study was conducted to evaluate the influence of cadmium, mercury and their combination on hematological parameters of Wistar rats. For this purpose, two different doses of each metal and their combination were administered orally for 28 days to six groups of five rats each. Two groups (A and B) were respectively exposed to CdCl₂ (0.25 and 2.5 mg/kg), two other groups (C and D) respectively received HgCl₂ (0.12 and 1.2 mg/kg) and the last two groups (E and F) were respectively treated with the combination of these two metals: (0.25 mg/kg    Cd + 0.12 mg/kg Hg) and (2.5 mg/kg Cd + 1.2 mg/kg Hg). The control group (G) received the same volume of distilled water. At the end of exposure, bodies of rats were weighed and the whole blood was collected by retro-orbital sinus method for analysis of hematological parameters. The results of this study show a significant decrease (p<0.05) in white blood cells (WBC) in the lot treated with the combination (0.25 mg/kg Cd + 012 mg/kg Hg) and also indicate a significant decrease (p<0.05) in WBC, red blood cells (RBC), hemoglobin concentration (HGB) and the mean corpuscular hemoglobin concentration (MCHC) with high levels of mercury (2.5 mg/kg) and the combination (2.5 mg/kg Cd + 1.2 mg/kg Hg). An increase in the number of platelet count (PLT) in all intoxicated lots was observed.

Key words: Cadmium, mercury, hematology, blood parameters, rats.

Abbreviation

Abbreviations: WBC, White blood cell count; RBC, red blood cells; HGB,hemoglobin concentration; HCT, haematocrit; MCH, mean corpuscular hemoglobin,MCV, volume of mean corpuscular erythrocyte; MCHC, mean corpuscular hemoglobin concentration; PLT, platelet count; LYM, number of lymphocytes; ROS,reactive oxygen species.