African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12506

Full Length Research Paper

Second derivative spectrophotometric determination of cyclophosphamide in pharmaceutical formulations

Nabil A. Fakhre1*, Hemn A. Qader 2 and Alaadin M. Naqishbandi3
1Chemistry Department, College of Education, Salahaddin University, Erbil, Iraq. 2Pharmaceutical Chemistry Department., College of Pharmacy, Hawler Medical University, Erbil, Iraq. 3Pharmacognosy Department, College of Pharmacy, Hawler Medical University, Erbil, Iraq.
Email: [email protected], [email protected]

  •  Accepted: 10 October 2013
  •  Published: 13 November 2013

Abstract

A new, simple, rapid, wide applicable range and reliable second derivative spectrophotometric method has been developed for determination of cyclophosphamide (CP) in bulk and pharmaceutical dosage forms. Calibration graph is linear in the concentration range of 25 - 200 μg/ml of CP with 10 μg/ml of detection limit and correlation coefficient of 0.9976. The precision and accuracy were acceptable depending upon the values of relative standard deviation and error percentage. Developed second derivative spectrophotometric method can be directly and easily applied for analyzing pure form and commercial pharmaceutical preparations of CP. The method was compared with a standard high performance liquid chromatography (HPLC) method and can be used for the quality control of pharmaceutical preparations in Erbil City.

 

Key words: Cyclophosphamide, derivative spectrophotometry.

Abbreviation

Abbreviations: CP, Cyclophosphamide; HPLC, high performance liquid chromatography; GC, gas chromatography; MEPS, micro-extraction packed sorbent.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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