Abstract

DNA extraction is an important step in molecular assays and plays a vital role in obtaining high-resolution results in gel-based systems, particularly in the case of cereals with high content of interfering components in the early steps of DNA extraction. Here a rapid mini-prep DNA extraction method, optimized for rice, which was achieved via some modifications in present DNA extraction methods, especially in first step of cell wall lyses and the use of cheap and frequent chemicals found in every laboratory is presented. Normal quality and quantity was obtained by the method. The PCR based assays also revealed the efficiency of the method. This method is applicable with both dry and fresh samples, does not require liquid nitrogen, and is easy, rapid and applicable in every laboratory.

Key words: PCR, DNA extraction, miniprep, rice.