African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12501

Full Length Research Paper

Antagonistic bioactivity of an endophytic bacterium isolated from Epimedium brevicornu Maxim

Rong-lin He1, Guo-ping Wang2, Xiao-Hong Liu1, Chu-long Zhang1* and Fu-cheng Lin1*
1State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Kaixuan Road 268, Hangzhou 310029, China. 2Dayang Chemical CO. Ltd., Chaoyang Road 22, Dayang town, Jiande, 311616, China.  
Email: [email protected], [email protected]

  •  Accepted: 02 July 2008
  •  Published: 19 January 2009

Abstract

Endophytic bacteria are one of the most potential biological control agents in plant disease protection. The aim of this work was to evaluate the antimicrobial activities of a strain of endophytic bacterium which was isolated from Epimedium brevicornuMaxim. The dual tests revealed that this endophytic bacterium strain displayed a wide-spectrum antimicrobial activity against 14 fugal phytopathogens and one bacterial phytopathogen; it especially strongly inhibited Alternaria alternata,Sclerotinia sclerotiorumVerticillium dahliaeBotrytis cinerea and Botrytis fabae. Identification of this strain based on morphology, physiological and chemical characteristics and 16S rRNA gene sequence analysis demonstrated that it belonged to the genus Phyllobacterium.

 

Key words: Endophytic bacteria, antimicrobial bioactivity, 16S rRNA gene.