Abstract

Ascorbate accumulation levels, distribution and key enzyme activities involved in synthesizing via Smirnoff-Wheeler pathway and recycling in different pear fruit tissues during development were studied. Results show that the ascorbate contents increased with the fruit development, and reached the highest titers in 30 days after anthesis (DAA), then decreased and maintained a level. The higher contents of ascorbate in the peel of pear fruit were observed, which results from a combination of higher activities of L-galactose dehydrogenase (GalDH) and L-galactono-1,4-lactone (GalLDH) involving ascorbate biosynthesis and higher dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR) activities used to recycle ascorbate. Exogenous feeding of ascorbate synthesis precursors demonstrated that the peel had stronger capability of de novo ascorbate biosynthesis via Smirnoff-Wheeler pathway and uronic acid pathway whereas the flesh and core had lower capability for ascorbate synthesis. These results suggest that the pear fruit is able to cause de novo ascorbate biosynthesis and the peel had higher capability for ascorbate biosynthesis than the flesh and core.

Key words: Pyrus pyrifolia, ascorbate, biosynthesis.

Abbreviation

 DAA, Days after anthesis; GalDH, L-galactose dehydrogenase;GalDH, L-galactose dehydrogenase; DHAR, dehydroascorbate reductase; MDHAR,monodehydroascorbate reductase; AsA, ascorbic acid.