Abstract

MS (Murashige and Skoog) media supplemented with benzylaminopurine (BAP) showed that the number of bud formation in shoot cultures of Musa acuminatacv.Berangan during the initiation stage increased proportionately with the concentrations used (11, 22 and 33 µM). However, the highest concentration of BAP (33 µM) simultaneously increased the formation of abnormal shoots. After the first apical bud appeared, explants were transferred to MS medium with lower concentrations of BAP either with or without indole acetic acid (IAA). Proliferation media supplemented with IAA showed enhanced shoot multiplication and elongation but did not help to reduce the abnormality index that occurred.

Key words: micropropagation, adventitious buds, banana, Musa acuminata

Abbreviation

PGR, Plant growth regulators; BAP, benzylaminopurine; IAA, indole acetic acid; MS, Murashige and Skoog; IBA, indole-3-butyric acid.