African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12502

Full Length Research Paper

Synthesis of the human VEGF165 gene based on overlap PCR and recombinant expression in stable transfected CHO cells

Jihong Zhang, Tianyun Wang*, Baosheng Yang, Yan Lin and Zhaoxi Li    
  Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Henan 453000, P. R. China.
Email: [email protected]

  •  Accepted: 25 May 2011
  •  Published: 16 January 2012

Abstract

 

Vascular endothelial growth factors (VEGFs) are a member of a family of structurally related proteins that mediate angiogenesis, and vascular maintenance. VEGF165 is an isoform and has been studied extensively because of its potential use in therapeutic angiogenesis. In this study, we synthesized the human VEGF165(hVEGF165) gene based on overlap PCR method and recombinant expressed in Chinese’s hamster ovary (CHO) cell. The coding sequence of hVEGF165 was synthesized successfully and the amounts of the recombinant protein could reach to 603.085 pg/ml. This rhVEGF165 protein could promote angiogenesis in chicken embryos. In conclusion, we established the stable expression system of recombinant human VEGF165 (rhVEGF165) protein in CHO cells.

 

Key words: Overlap extension PCR (OE-PCR), vascular endothelial growth factor (VEGF), Chinese’s hamster ovary (CHO) cell, recombinant protein.

Abbreviation

 

VEGFs, Vascular endothelial growth factors; CHO, Chinese’s hamester ovary; hVEGF, human vascular endothelial growth factor; rhVEGF,recombinant human vascular endothelial growth factor; PLGF, placental growth factor.

 

Copyright © 2025 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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