African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12500

Full Length Research Paper

High production of fructooligosaccharides by levansucrase from Bacillus subtilis natto CCT 7712

Patrícia Bittencourt da Silva*
  • Patrícia Bittencourt da Silva*
  • Department of Biochemistry and Biotechnology, State University of Londrina, Rodovia Celso Garcia Cid, PR 445 Km 380, Londrina, Paraná State, Brazil.
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Dionísio Borsato
  • Dionísio Borsato
  • Department of Chemistry, State University of Londrina, Rodovia Celso Garcia Cid, PR 445 Km 380, Londrina, Paraná State, Brazil.
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Maria Antonia Pedrine Colabone Celligoi
  • Maria Antonia Pedrine Colabone Celligoi
  • Department of Biochemistry and Biotechnology, State University of Londrina, Rodovia Celso Garcia Cid, PR 445 Km 380, Londrina, Paraná State, Brazil.
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  •  Received: 13 March 2014
  •  Accepted: 16 June 2014
  •  Published: 02 July 2014

Abstract

Fructooligosaccharides (FOSs) are fructose oligomers known as prebiotics that beneficially affect the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria in the colon, and thus improving host’s health. FOS can be produced by the action of fructosyltranferases from microorganisms, including the Bacillus subtilis natto. The objective of the present study was applying response surface methodology in order to optimize the FOS production by levansucrase from B. subtilis natto CCT 771. The variables evaluated were sucrose concentration, pH and temperature. From the analysis of the results, the significant variables were pH and sucrose concentration (p < 0.05). Levansucrase of B. subtilis natto synthesized fructooligosaccharides in all experimental conditions, showing its potential for use in an industrial process of producing FOS from sucrose. The use of response surface methodology allowed the determination of the concentration of sucrose (334 g.L-1), pH (6.0) and temperature (45.8°C) for a maximum yield of 54.86 g.L-1 FOS.

 

Key words: Fructooligosaccharides, Bacillus subtilis, levansucrase, prebiotics.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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