African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12502

Full Length Research Paper

Molecular diagnosis of recent suspected outbreaks of peste des petits ruminants (PPR) in Yola, Adamawa State, Nigeria

  H. G. Ularamu1*, O. A. Owolodun2, T. Y. Woma1, B. J. Audu2, G. B. Aaron1, S. C. Chollom1 and D. Shamaki1  
  1Morbillivirus Research Laboratory, Viral Research Division, National Veterinary Research Institute, P.M.B. 01, Vom 930001, Nigeria. 2Department of Biochemistry and Applied Molecular Biology, National Veterinary Research Institute, P.M.B. 01, Vom 930001, Nigeria.
Email: [email protected]

  •  Accepted: 04 March 2011
  •  Published: 16 January 2012

Abstract

 

Peste des petits ruminants (PPR) is an economically important viral disease of goats and sheep. It is endemic in Nigeria and was first described in West Africa in the 1940s. Clinically, PPR can be confused with other infections such as pasteurellosis or contagious ecthyma and mild strain of rinderpest in small ruminants. Effective implementation of control measures for PPR requires that a proper and rapid diagnosis of the disease is made. Therefore, the use of reverse transcriptase polymerase chain reaction to analyze suspected field samples collected from sheep and goats in Yola, Adamawa State has helped to give urgent diagnosis that was needed to effectively control the spread of the disease. The samples collected on filter paper were suitable source of the viral RNA.

 

Key words: Peste des petits ruminants, Morbillivirus, reverse transcriptase polymerase chain reaction, diagnosis.