African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12502

Full Length Research Paper

GFLV coat protein constructs based on local isolates from the Czech Republic

  Josef Vlasák1*, JindÅ™ich BÅ™iza1, Hana Niedermeierová1, Daniela Pavingerová1, Aleš Eichmeier2, Miroslav Baránek2 And Miroslav Pidra2  
  1Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences Of the Czech Republic, ÄŒeské BudÄ›jovice, Czech Republic. 2Faculty of Horticulture in Lednice, Mendeleum – Institute of Genetics, Mendel University in Brno, Lednice, Czech Republic.
Email: [email protected]

  •  Published: 16 January 2012

Abstract

 

Coat protein genes of grapevine fanleaf virus local strains isolated in South-Moravia, Czech Republic were sequenced, and artificial coat protein gene was designed and synthesized. It retains typical sequence features of local strains but is free of mRNA destabilizing sequences. Three variants of the synthetic gene were cloned intoAgrobacterium plant expression vector and their function was tested after potato protoplasts transformation, assaying transient mRNA and coat protein production.

 

Key words: Grapevine fanleaf virus, Vitis vinifera, sequence, protoplast expression, resistance.

Abbreviation

GFLV, Grapevine fanleaf virus; CP, coat protein; GMO, genetically modified organism.

Copyright © 2025 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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