African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Full Length Research Paper

Non liquid nitrogen-based-method for isolation of DNA from filamentous fungi

Osama E. Amer1, Mohamed. A. Mahmoud1,2*, AbdEl-Rahim M. A. El-Samawaty1,2 and Shaban R. M. Sayed1
1Botany and Microbiology Department, College of Science, King Saud University, Riyadh, P. O. Box: 2455, Riyadh 11451, Kingdom of Saudi Arabia. 2Agricultural Research Center, Plant Pathology Research Institute, Giza, Egypt.
Email: [email protected]

  •  Accepted: 12 August 2011
  •  Published: 24 October 2011

Abstract

A simple, efficient, reliable and cost-effective method for isolation of total genomic DNA from fungi, suitable for polymerase chain reaction (PCR) amplification and other molecular applications was described. The main advantages of the method are: (1) does not require the use of liquid nitrogen for preparation of fungi DNA; (2) the mycelium is directly recovered from Petri-dish cultures; (3) the quality and quantity of DNA obtained are suitable for molecular assays; (4) the technique is rapid and relatively easy to perform; (5) it can be applied to filamentous fungi from soil as well as from a fungi from other environmental sources; and (6) it does not require the use of expensive and specialized equipment or hazardous reagents.This method does not require liquid nitrogen for fixation, grinding or storage at -80°C, making it advantageous over other common protocols.

 

Key words: Genomic DNA extraction, filamentous fungi, polymerase chain reaction (PCR) amplification.