African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Full Length Research Paper

Isolation and characteristic of an aerobic denitrifier with high nitrogen removal efficiency

Shu-Cheng Liang, Min Zhao*, Lei Lu, Chun-Lei Wang, Li-Yan Zhao and Wen-Jin Liu
College of Life Sciences, Northeast Forestry University, No. 26, He-Xing Road, Harbin, Heilongjiang 150040, P. R. China.
Email: [email protected]

  •  Accepted: 21 July 2011
  •  Published: 12 September 2011

Abstract

 

Paracoccus denitrificans DL-23, isolated from aerobic domesticated activated sludge, was demonstrated to have high ability of denitrification and heterotrophic nitrification under aerobic condition. After optimization (succinate, COD/N 10, 37°C, 160 rpm), DL-23 removed 420 and 860 mg/l NO3- -N within 36 to 60 h of growth, respectively. DL-23 also removed 380 mg/l NH4+-N within 24 h with ammonia as nitrogen source. The maximum removal rate was 30.3 mg/l·h. Meanwhile, DL-23 exhibited aerobic nitrite reduction ability with 658 mg/l NO2--N within 48 h.

 

Key words: Aerobic denitrification, heterotrophic nitrification, nitrogen removal,Paracoccus denitrificans.