African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12500

Full Length Research Paper

Ten (10) M Ammonium acetate is an efficient molecular concentration for the extraction of genomic DNA from small insects used for rolling circle amplification

S. M. Muturi1, 2*, L. S. Karanja3, F. N. Wachira4 and M. C. Wambulwa2
1Department of Biological Sciences, Meru University of Science and Technology, P. O. Box 972, Meru, Kenya. 2Department of Biochemistry and Molecular Biology, Egerton University, P. O. Box 536, Egerton, Kenya. 3Kenya Agricultural Research Institute (KARI)-Njoro, P.O Box Private bag, Kenya. 4Association for Strengthening Agricultural Research in Eastern and Central Africa (ASARECA), P. O. Box 765, Entebbe, Uganda.
Email: [email protected]

  •  Accepted: 27 August 2013
  •  Published: 25 September 2013

Abstract

Most of the published methods for DNA isolation from vectors (insects) are not always effective in mealy-bugs, aphids and leafhoppers because they have high amount of polyphenols in their body systems. A simple, rapid, reliable labor-effective and carcinogenic compound-free salting out method for isolation of high molecular DNA from these insects is described. The ratio of OD260/OD280 ranged between 1.8 to 2.0. The method was successfully applied to Rolling Circle Amplification and in restriction analysis, indicating the removal of the common inhibitors.

 

Key words: Pseudococcidae, Aphididae, Auchenorryncha, DNA extraction, rolling circle amplification, restriction analysis.

Copyright © 2025 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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