African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12501

Full Length Research Paper

Free radical scavenging activities of pigment extract from Hibiscus syriacus L. petals in vitro

  Mingjiang Geng1*, Mingxin Ren2, Zhenling Liu1 and Xiaojun Shang3  
  1Department of Chemistry, Xinxiang Medical University, Xinxiang 453003, China. 2Department of Anatomy, Xinxiang Medical University, Xinxiang 453003, China. 3College of Pharmacy, Xinxiang Medical University, Xinxiang 453003, China.
Email: [email protected]

  •  Published: 05 January 2012

Abstract

 

The macroporous resin adsorption method was used to purify the pigment fromHibiscus syriacus L. petals. Through the comparison of the adsorption and desorption rates of six types of macroporous resins including AB-8, S-8, NKA-9, DM-130, D101 and 860021 to the pigment, 860021 resin was selected as the most appropriate resin to purify the pigment. The antioxidant capacities of the pigment extract (PE) were evaluated through in vitro experiments using hydroxyl radical scavenging assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay and lipid peroxidantion (LPO) inhibition capacity assay induced by Fe2+-H2O2. Total flavonoid content (TFC) of PE was determined using the colorimetric methodology and total phenolic content (TPC) using Folin–Ciocalteu reagent. PE produced significant antioxidant activity. In addition, PE demonstrated higher TFC and TPC of 63.4±1.8 mg rutin equivalents/g and 172.6±2.4 mg gallic acid equivalents/g, respectively. This study suggests that H. syriacus L. petal can be used potentially as a source of natural antioxidants.

 

Key words: Macroporous resin, Hibiscus syriacus L., pigment, free radical.

Abbreviation

PE, Pigment extract; DPPH, 1,1-diphenyl-2-picrylhydrazyl; LPO,lipid peroxidation; TPC, total phenolic content; TFC, total flavonoid content; MDA,malondialdehyde.