African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Full Length Research Paper

Genetic characterization of angular leaf spot resistance in selected common bean landraces from Tanzania

George Muhamba Tryphone*
  • George Muhamba Tryphone*
  • Department of Crop Science and Production, Faculty of Agriculture, Sokoine University of Agriculture (SUA), P. O. Box 3005, Chuo Kikuu, Morogoro, Tanzania.
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Luseko Amos Chilagane
  • Luseko Amos Chilagane
  • Department of Crop Science and Production, Faculty of Agriculture, Sokoine University of Agriculture (SUA), P. O. Box 3005, Chuo Kikuu, Morogoro, Tanzania.
  • Google Scholar
Susan Nchimbi-Msolla
  • Susan Nchimbi-Msolla
  • Department of Crop Science and Production, Faculty of Agriculture, Sokoine University of Agriculture (SUA), P. O. Box 3005, Chuo Kikuu, Morogoro, Tanzania.
  • Google Scholar
Paul Mbogo Kusolwa
  • Paul Mbogo Kusolwa
  • Department of Crop Science and Production, Faculty of Agriculture, Sokoine University of Agriculture (SUA), P. O. Box 3005, Chuo Kikuu, Morogoro, Tanzania.
  • Google Scholar


  •  Received: 18 July 2014
  •  Accepted: 23 September 2015
  •  Published: 28 October 2015

Abstract

Angular leaf spot disease (ALS) caused by Pseudocercospora griseola is one of the most important bean diseases in Tanzania. The bean landraces Beti-10, Nanka, Nanavala and Nkanamna used in this study have been identified as resistant to ALS but the nature of inheritance and mechanisms of resistance against ALS in those potential sources has not been elucidated. This information is crucial and a necessary first step for a successful breeding programme. The objective of this work was to study the inheritance of ALS resistance in those landraces and to identify the mechanisms of genetic resistance using Simple Sequence Repeat (SSR) markers. Crosses were made between resistant bean landraces and a susceptible bean cv Kablanketi. The parents, F1, F2 and backcrosses derived plants were used for inheritance studies and for molecular marker screening using 30 SSR markers. Results indicate that, a single dominant gene control resistance against ALS in each of the four landraces; also the SSR marker Pv-ag004 was found to be polymorphic between Beti-10 and Kablanketi and linked to the disease resistance. The resistance were validated by checking the F2 population of the cross between Kablanketi × Beti-10. Therefore, since marker Pv-ag004 is polymorphic and linked to ALS resistance, the Beti-10 landrace might be a potential source of ALS resistance. However, a detailed study with more markers need to be done on these landraces with a view to opening the possibilities of identifying new markers linked to ALS resistance and mapping of genes associated with resistance to ALS.

Key words: Phaseolus vulgaris, Inheritance, ALS, SSR, Pseudocercospora griseola.

Abbreviation

ALS, Angular leaf spot; SSR, simple sequence repeat; PCR, polymerase chain reaction.