African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12500

Full Length Research Paper

Investigation of some important phytochemical, nutritional properties and toxicological potentials of ethanol extracts of Newbouldia laevis leaf and stem

Anaduaka, Emeka G.1*, Ogugua, Victor N.1, Egba, Simeon I.2 and Apeh, Victor O.1
1Department of Biochemistry, University of Nigeria, Nsukka, Nigeria. 2Department of Biochemistry, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria.
Email: [email protected], [email protected]

  •  Accepted: 02 September 2013
  •  Published: 02 October 2013

Abstract

The pytochemicals, nutritional and toxicological potentials of the ethanol extracts of the leaf and stem of Newbouldia laevis was investigated in this study. The percentage yields of N. laevis ethanol leaf and stem extracts were found to be 7.44 and 3.30% (w/w), respectively. The preliminary phytochemical screening showed that ethanol leaf and stem extracts contains alkaloids, flavonoids and tannins. The quantitative phytochemical analysis showed that the leaf and stem extracts contained respectively: alkaloids (14.74­­ ± 0.06 and 6.27­­ ± 0.0 mg/g), flavonoids (15.51 ± 0.04 and 5.18 ± 0.04 mg/g), cardiac glycosides (6.77 ± 0.02 mg/g), tannins (1.74 ± 0.11 mg/g), saponins (4.07 ± 0.06 mg/g), steroids (41.72 ± 0.02 mg/g) and terpenoids (8.67 ± 0.09 mg/g). The following amounts of vitamins and minerals were found in the leave and stem-bark extracts, respectively; vitamin A (5.19­ ­± 0.00 and 3.01 ­­± 0.00 mg/100 g), vitamin C (2.35 ­­± 0.55 and 1.05­ ­± 0.08 mg/100 g) and vitamin E (9.33­­ ± 0.02 and 4.08­ ± 0.11 mg/100 g); minerals: Mg (76.12 ± 0.04 and 54.25 ± 0.04 mg/100 g), Fe (16.84 ± 0.06 and 1.19 ± 0.03 mg/100 g) and Se (3.08 ± 0.03 and 0.29 ± 0.07 mg/100 g). The acute toxicity test of the ethanol leaf and stem extracts showed no toxicity up to 5000 mg/kg body weight.

 

Key words: Newbouldia laevis, phytochemical properties, vitamins, minerals, toxicity.