Abstract

Fraxinus angustifolia is used for afforestation and for production of both valuable timber and manna, a substance with pharmacological applications. The aim of this research was to establish the optimized condition for cryopreservation of F. angustifolia seeds, in order to protect the germplasm of this species. Germination percentage and mean time of germination of non-treated seeds (control) and liquid nitrogen-treated seeds were established in water or in 10^{-6 M gibberellic acid (GA}₃). The seeds could be cryopreserved with 3% of moisture content (MC) and germinated easily in water (70.0 ± 5.0%), while seeds cryopreserved with 6% MC showed a physiological dormancy. This state could be efficiently removed by addition of GA₃, which increased germination to 76.0 ± 2.4%. Liquid nitrogen did not impair embryo viability, allowing a successful cryopreservation of seeds. The results are particularly useful for both germplasm conservation programs and in narrow leaved ash nurseries.

Key words: Fraxinus angustifolia, germination, germplasm conservation, seed cryopreservation.

Abbreviation

 Cryo, Cryopreserved seeds; GA₃, gibberellic acid; LN, liquid nitrogen;MC, moisture content; MTG, mean time of germination; NT, non-treated seeds; TTC,2,3,5-triphenyl tetrazolium chloride.