African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12502

Full Length Research Paper

Laboratory scale production of the human recombinant iduronate 2-sulfate sulfatase-Like from Pichia pastoris

Henry A. Córdoba-Ruiz1, Raúl A. Poutou-Piñales1,3,  Olga Y. Echeverri-Peña1,  Néstor A. Algecira-Enciso2, Patricia Landázuri1,4, Homero Sáenz1,5 and Luis A. Barrera-Avellaneda1*
  1Instituto de Errores Innatos del Metabolismo, Facultad de Ciencias, Pontificia Universidad Javeriana. Bogotá D.C., Colombia. 2Universidad Nacional de Colombia. 3Laboratorio de Biotecnología Aplicada, Grupo de Biotecnología Ambiental e Industrial, Departamento de Microbiología. Facultad de Ciencias, Pontificia Universidad Javeriana. Bogotá D.C., Colombia. 4Laboratorio de Bioquímica y Genética. Facultad de las Ciencias de la Salud Universidad del Quindío, Armenia, Colombia. 5Unidad de Biología Celular y Microscopía, Decanato de Ciencias de la Salud, Universidad Centroccidental Lisandro Alvarado, Barquisimeto, Venezuela.
Email: [email protected]

  •  Accepted: 30 March 2009
  •  Published: 04 May 2009

Abstract

 

Clone IDS28 of the yeast Pichia pastoris expressing the human iduronate 2-sulfate sulfatase-Like (hIDS-Like) was employed for low-scale production of the recombinant enzyme in a saline culture media without phosphate.  The biological activity found was between 7.3 and 29.5 nmol h-1 mg-1 of total protein. It is about 1.73 to 7 times higher than the result obtained with the same clone in shake flask culture.

 

Key words: Pichia pastoris, iduronate 2-sulfate sulfatase, monod model, Hunter syndrome, methanol induction.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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