African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Full Length Research Paper

Population of bottleneck and microsatellite: An Analysis Based on genetic diversity of Wild Tiger Shrimp Penaeus monodon (Fabricius) in Malaysia

Reza Nahavandi1,2, Parivash Hafezamini3, Hassan Moeini4, Mohammad Zareian Jahromi5 and Mariana Nor Shamsudin1
1Laboratory of Marine Biotechnology, Institute of Bioscience, University Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia. 2Iranian Fisheries Research Organization (I.F.R.O.), P.O. Box: 14155-6116 Tehran, Iran. 3Islamic Azad University, Rasht Branch, Educational Complex Bridge Talysh, Lake Gate, Rasht, Iran. 4Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, University Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia. 5Department of Biology, Islamic Azad University, Jahrom Branch, Jahrom, Iran.
Email: [email protected] or [email protected]

  •  Accepted: 21 October 2011
  •  Published: 23 November 2011

Abstract

In the present study, six microsatellite markers, namely, CSCUPmo1, CSCUPmo2, CSCUPmo3, CSCUPmo4, CSCUPmo6 and CSCUPmo7 were used to monitor genetic variation in the wild tiger shrimp, Penaeus monodon (Fabricius). The number of alleles examined by all the microsatellites yielded the monitoring ranges of 2 to 6 alleles. Moreover, the highest and lowest heterozygosity were found to be at a significant level of 0.33 in CSCUPmo1 and at a significant level of 0.47 in CSCUPmo2, respectively. These observations indicated the existence of substantial genetic diversity in P. monodon in Malaysia. None normal 'L' shaped distribution of mode-shift test, significant heterozygote excess on the basis of different models, as revealed from Sign, standardized differences and Wilcoxon sign rank tests as well as significant M ratio value suggested that there was recent bottleneck in the existing population, providing important information for shrimp breeders.

 

Key words: Genetic bottleneck, Penaeus monodon, microsatellite, heterozygote.