African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12501

Full Length Research Paper

Effect of pH, temperature and water activity on the inhibition of Botrytis cinerea by Bacillus amyloliquefaciens isolates

  Hamdache Ahlem1, Ezziyyani Mohammed2, Alain Badoc3 and Lamarti Ahmed1*  
  1Département de Biologie, Faculté des Sciences, Equipe de Biotechnologies Végétales. M’hannech II, BP 2121 93002 Tétouan, Maroc. 2Département de Biologie, Faculté Polydisciplinaire de Khouribga, Université Hassan Premier Settat, El Hay El Jadid, El Byout, BP 145 (OCP), Khouribga principale, 25000, Maroc. 3GESVAB-EA 3675, UFR des Sciences Pharmaceutiques, Université Bordeaux Segalen, ISVV, 210 Chemin de Leysotte, CS 50008, 33882 Villenave-d’Ornon, France.
Email: [email protected]

  •  Accepted: 12 August 2011
  •  Published: 31 January 2012

Abstract

 

A total of 321 bacterial strains were isolated from the rhizosphere of healthy strawberry (Fragaria X ananassa Duch.) plants harvested from different places in Zlaoula area (Larache, Morocco). They were screened by dual testing for in vitroantagonism towards Botrytis cinerea (Bt7). Nine antagonistic strains were retained. Their effectiveness was evaluated according to temperature (5 to 30°C), pH (4 to 10) and water activity (aw, 0.80 to 1). Low values of water activity and acid pH were unfavourable for the growth inhibition. All the isolates were identified to belong toBacillus amyloliquefaciens.

 

Key words: Antifungic activity, water activity, Bacillus amyloliquefaciens, Botrytis cinerea, pH, temperature.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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