African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12502

Full Length Research Paper

Physicochemical and microbiological characterization of linolenic acid-rich oils from seeds of two tropical plants: Corchorus olitorius L. and Hibiscus sabdariffa L.

Lessoy Zoué1, Micael Bédikou1, Betty Faulet2, Jean Gonnety2 and Sébastien Niamké1*
  1Laboratoire de Biotechnologies, Filière Biochimie-Microbiologie de l’Unité de Formation et de Recherche en Biosciences de l’Université de Cocody, 22 BP 582 Abidjan 22, Côte d’Ivoire. 2Laboratoire de Biochimie et Technologie des Aliments de l’Unité de Formation et de Recherche en Sciences et Technologie des Aliments de l’Université d’Abobo-Adjamé, 02 BP 801 Abidjan 02, Côte d’Ivoire.
Email: [email protected]

  •  Accepted: 23 November 2011
  •  Published: 15 May 2012

Abstract

 

his work was carried out to determine the potential applications of Corchorus olitorius and Hibiscus sabdariffa oil seeds by investigating their physicochemical and microbiological characteristics. Physicochemical parameters of the extracted oils were respectively as follow: refractive index (1.46 and 1.45), acid value (3.74 ± 1.62 and 2.80 mg KOH/g), peroxide value (6.67 ± 0.58 and 5.33 ± 0.60 meq O2/kg), iodine value (132.54 ± 1.22 and 118.57 ± 1.22 g I2/100 g), saponification value (183.63 ± 3.24 and 189.23 ± 1.62 mg KOH/g), unsaponifiable matter (0.88 ± 0.04 and 0.85 ± 0.05 %), vitamin A (0,45 ± 0.01 and 0.60 ± 0.01 mg/g), cholesterol (0.00 mg/g) for C. olitoriusand H. sabdariffa respectively . Absorbances of the two oils decreased abruptly in the range of UV-B and UV-A wavelengths. C. olitorius and H. sabdariffa seeds showed relatively high content of linolenic acid (about 29 and 37% of total fatty acids) with extremely low n-6/n-3 ratio (0.26 and 0.46). Microbiological analysis revealed that the crude extracted oilseeds were pathogenic bacteria free and the lipolytic bacteria counted belonged to Micrococcus genus. All these interesting characteristics should arouse attention for the usage of these oilseeds in food, pharmaceutical and cosmetic industries.

 

Key wordsCorchorus olitorius, Hibiscus sabdariffa, seed oils characterization, linolenic acid, lipolytic bacteria.