African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Full Length Research Paper

Cloning and molecular characterization of a copper chaperone gene (HbCCH1) from Hevea brasiliensis

Hui-Liang Li1, Dong Guo1, Wei-Min Tian2 and Shi-Qing Peng1*      
1Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, People’s Republic of China. 2Key Laboratory of Rubber Biology, Ministry of Agriculture, Institute of Rubber, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, People’s Republic of China.  
Email: [email protected]

  •  Accepted: 21 March 2011
  •  Published: 20 June 2011

Abstract

The cDNA encoding a copper chaperone, designated as HbCCH1, was isolated fromHevea brasiliensisHbCC1 was 589 bp long containing a 261 bp open reading frame encoding a putative protein of 86 amino acids, flanked by a 103 bp 5’UTR and a 225 bp 3’UTR. The predicted molecular mass of HbCCH1 was 9.2 kDa, with an isoelectric point (pI) of 5.13. The HbCCH1 share the conserved N-terminal metal-binding domain (MXCXXC) and a lysine-rich C-terminus. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis revealed that HbCCH1 was constitutively expressed in all the tested tissues. HbCCH1 transcripts were accumulated at relatively low levels in the flower, bud and leaves, while HbCCH1 transcripts were accumulated at relatively high levels in the latex. The transcription of HbCCH1 in the latex was induced byjasmonate.

 

Key words: Copper chaperone, Hevea brasiliensis, latex.

Abbreviation

 CCH, Copper chaperone; JA, jasmonate; ORF, open reading frame;RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcriptase polymerase chain reaction; SOD, superoxide dismutase; UTR, untranslated region.