African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Full Length Research Paper

Examination of genetic diversity in common bean (Phaseolus vulgaris L.) using random amplified polymorphic DNA (RAPD) markers

Asifa Bukhari
  • Asifa Bukhari
  • Division of Genetics and Pant Breeding Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir Shalimar campus, Srinagar -191 121, India.
  • Google Scholar
M. A. Bhat
  • M. A. Bhat
  • Division of Genetics and Pant Breeding Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir Shalimar campus, Srinagar -191 121, India.
  • Google Scholar
Mushtaq Ahmad*
  • Mushtaq Ahmad*
  • Division of Genetics and Pant Breeding Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir Shalimar campus, Srinagar -191 121, India.
  • Google Scholar
Noorul Saleem
  • Noorul Saleem
  • Division of Genetics and Pant Breeding Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir Shalimar campus, Srinagar -191 121, India.
  • Google Scholar


  •  Received: 12 October 2014
  •  Accepted: 02 February 2015
  •  Published: 11 February 2015

Abstract

To study the pattern of genetic diversity in 45 genotypes of common bean, 19 RAPD primers were used. Of 253 bands produced, 236 bands (94.22%) were polymorphic in which maximum number (20 polymorphic bands) were observed in the profiles of the primer OPB-07. Highest PIC value (0.79) was observed for the primers OPG-14 and OPE-1, whereas lowest PIC value (0.34) was recorded for the primer OPA-11 with an average PIC value of 0.54. Similarly, highest effective multiplex ratio value (0.11) was scored for the primers OPA-04 and OPG-6 and the lowest value (0.05) was recorded for the primer OPB-07. Primer OPG-14 and OPE-1 exhibited the highest marker index value (0.078) and the lowest value (0.024) was recorded for OPA-11. Pair-wise genetic similarity coefficients of genotypes varied from 0.56 to 0.92 with an average of 0.70 amongst 45 genotypes. The maximum similarity coefficient (0.92) was noticed between G-9 and G-8. Cluster analysis separated 45 genotypes into seven major clusters which were further grouped into various sub-clusters. Among these seven clusters, the maximum number of genotypes (12) were recorded in cluster I, while cluster VII formed a mono-genotypic cluster. 
 
Key words: Genetic diversity, polymorphism, cluster analysis, random amplified polymorphic DNA markers (RAPD), common bean.