African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12504

Full Length Research Paper

Optimized integration of T-DNA in the taxol-producing fungus Pestalotiopsis malicola

  Xiaoran Hao, Yuan Ji, Shourong Liu, Jiannan Bi, Qing Bi, Jiao Pan and Xudong Zhu*
  National Key Program of Microbiology, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, PR China.  
Email: [email protected]

  •  Accepted: 04 November 2011
  •  Published: 12 January 2012

Abstract

 

We previously reported a taxol-producing fungus Pestalotiopsis malicola. There, wedescribed the transformation of the fungus mediated by Agrobacterium tumefaciens.T-DNA carrying the selection marker was transferred into the fungus and randomly integrated into the genome as shown by Southern blotting. Approximately 66% of the transformants obtained a single copy of T-DNA. Conditions of transformation were optimized. The method can be used for taxol-related genes searching and manipulation in P. malicola. This is the first report of T-DNA-mediated transformation in taxol-producing fungi.

 

Key words: T-DNA, random insertional mutagenesis, taxol, Pestalotiopsis.

Copyright © 2025 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 11 No. 4
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES