African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 10 January 2012; 11(3)

January 2012

cDNA sequence and tissue expression analysis of glucokinase from liver of grass carp (Ctenopharyngodon idella)

    A full-length cDNA coding glucokinase (GK) was cloned from the liver of grass carp (Ctenopharyngodon idella) by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The cDNA obtained was 2066 bp exclusive of poly (A) residues with a 1431 bp open reading frame encoding 476 amino acids. The GK protein has a calculated molecular...

Author(s):   Cheng Han-liang, Ji Nan-jing, Peng Yong-xing, Shen Xin, Xu Jian-he, Dong Zhi-guo and Wu Chen-chen    

January 2012

Cloning and expression of porcine SRPK1 gene

  Protein SRPK1 acts as a crucial element in the pre-initiation complex of transcription, which play an important role in the regulation procession of gene expression. This study was carried out in order to explore the genetic characteristic of SRPK1 in pigs.SRPK1 gene came from Yorkshire, a pig that was cloned by real time polymerase chain reaction (RT-PCR), yet coding sequence and partial...

Author(s):   Guang-xin, E., Di Liu, Dong-Jie Zhang, Xiu-Qin Yang and Ji-Yuan Zhu  

January 2012

Molecular cloning and expression analysis of an Mn-superoxide dismutase gene in sugarcane

  Superoxide dismutases (SODs) play an important role in stress-tolerance in plants. In this study, for the first time, a full-length cDNA sequence of MnSOD gene, termed asSc-MnSOD (GenBank accession number: GQ246460), was obtained in sugarcane. Sequence analysis revealed that Sc-MnSOD gene was 919 bp long, including a 702 bp ORF, the 5’ UTR of 99 bp and 3’UTR of 118 bp. It...

Author(s):   Youxiong Que, Jinxian Liu, Liping Xu, Jinrong Guo and Rukai Chen

January 2012

Using innovation platforms to scale out soil acidity-ameliorating technologies in Dedza district in central Malawi

  Soil acidity is a serious constraint in crop production in some parts of Malawi, particularly in high rainfall and high altitude areas. A learning platform was established in Bembeke Extension Planning Area (EPA) (14° 21′ E and 34° 21′ S, 1650 masl, normal annual rainfall of 1300 to 1500 mm) in Dedza District to scale out lime applications as amendments to low pH soils. In 2006/2007 and...

Author(s):   V. H. Kabambe, A. D. C. Chilimba, A. Ngwira, M. Mbawe, G. Kambauwaand P. Mapfumo

January 2012

Identification and characterization of a Bacillus subtilis strain TS06 as bio-control agent of strawberry replant disease (Fusarium and Verticilium wilts)

  Replant disease is a major limitation for strawberry production in greenhouse. Bio-control may be a good way to cope with the replant diseases. Here, we report identification and characterization of a bacterial strain TS06 that may be used as a bio-control agent against the replant diseases in strawberry. TS06 was identified as a new strain of Bacillus subtilis based on the homology of its 16S rRNA...

Author(s):   Yunting Zhang#, Tengfei Fan#, Wensuo Jia, Wen Zhang, Qizhi Liu, Bingbing Li and Lusheng Zhang  

January 2012

Cloning and expression analysis of a LFY homologous gene in Chinese jujube (Ziziphus jujube Mill.)

  LEAFY (LFY) homologous genes are necessary for the transition from vegetative to reproductive development in flowering plants. The full-length cDNA of a LFY homologwas successfully isolated from floral buds of Chinese Jujube (Ziziphus jujube Mill.) by degenerate reverse transcriptase-polymerase chain reaction (RT-PCR), rapid amplification of cDNA ends (RACE), and Blastn analysis....

Author(s):   Duan Jinghua, Li Fangdong, Du Hongyan, Zhang Lin, Yuan Deyi, Feng Yanzhi, Long Hongxu and Ma Lvyi    

January 2012

Microbial activity of soil during the inoculation of soya bean with symbiotic and free-living nitrogen-fixing bacteria

Soya bean has a great importance in the organic production due to its ability to coexist with nitrogen-fixing bacteria of the genus Bradyrhizobium. The soya bean seed inoculated with the symbiotic nitrogen-fixing bacteria Bradyrhizobium japonicum and with a mixture of nitrogen-fixing bacteria was observed in this study. The following highly efficient strains of free nitrogen-fixing...

Author(s): Gorica Cvijanović, Gordana Dozet, Vojin Djukić, Snežana ĐorÄ‘ević and Goran Puzić

January 2012

Regenerability of elite tropical maize (Zea mays L.) inbred lines using immature zygotic embryo explants

Five elite tropical maize inbred lines; CML395, CML443, CML442, MAS [MSR/312]-117-2-2-1-B-5-B) and CML216 as a control, were evaluated for their regenerability making use of calli derived from immature zygotic embryos. Murashige and Skoog basal salts supplemented with 1.0, 1.5, 2.0 and 2.5 mg/L 2,4-dichlorophenoxyacetic acid were used to induce callus. Callus induction frequency and formation of embryogenic callus...

Author(s): Leta Tulu Bedada, Miccah Songelael Seth, Steven Maina Runo, Wondyifraw Tefera and Machuka Jesse

January 2012

Silicon mediated biochemical changes in wheat under salinized and non-salinzed solution cultures

Silicon (Si) can alleviate salinity damage, a major threat to agriculture that causes instability in wheat production. We report on the effects of silicon (150 mg L-1) on the morphological, physiological and biochemical traits in wheat (Triticum aestivum L.) cultivars (salt sensitive; Auqab-2000 and salt tolerant; SARC-5) differing in salt tolerance under saline (10 dS m-1) and non-saline (2 dS m-1) hydroponic...

Author(s): Anser Ali, Shahzad M. A. Basra, Javaid Iqbal, Safdar Hussain, M. N. Subhani, Muhammad Sarwar and Ahmad Haji  

January 2012

Pollen morphology and variability of Tulipa hungarica Borb

Pollen morphology of the endemic species Tulipa hungarica Borb was investigated with the goal of diversity protection and conservation. Morphology of pollen was evaluated using a scanning electron microscopy. Pollen grain was monosulcate with perforate striate exine surface. Also, pollen of T. hungarica showed differences in size, shape, and staining intensity after aceto-orceine treatment. The...

Author(s): Dušica Ćalić, Nina Devrnja, Jelena Milojević, Snežana Zdravković-Korać, Ljiljana Tubić, Milutin S. Djuričković and Branka Vinterhalter  

January 2012

Influence of salicylic acid on growth and some biochemical parameters in a C4 plant (Panicum miliaceum L.) under saline conditions

  Salt stress is considered as a restricting factor for plant products. Therefore, many compounds were applied to minimize the harmful effects of this stress. In this study, millet plants (Isfahan cultivar) were sprayed with 0.1, 0.3, 0.5 and 1 mM salicylic acid and then treated with 0, 150, 225 and 325 mM NaCl. Our results show that in plants not sprayed with salicylic acid but treated with NaCl,...

Author(s): Enteshari, Shekoofeh and Sharifian, Shahla  

January 2012

Heavy metal ions adsorption by suspended particle and sediment of the Chalus River, Iran

  Nowadays, it is important to evaluate the self-purifying capacity of rivers because of the different kinds of pollutants discharged into them. Important kind of pollutants and heavy metals exist in wastewaters industries. When the Sorb Dona mine is placed in Upper Chalus River, in the west of Mazandaran, products of mine wastewater are discharged into the river, making this important and protective river to...

Author(s):   A. Hasannattaj Jelodar , H. Amini Rad, B. Navaiynia and M. A. Zazouli  

January 2012

Antioxidative enzyme activities in the leaves and callus tissues of salt-tolerant and salt-susceptible melon varieties under salinity

  The response of the antioxidant system to salt stress was studied in the leaves and callus tissues of 4 Turkish melon varieties (Cucumis melo L.) Besni, Yuva, Midyat, and Semame and a melon cultivar Galia C8. The antioxidant capability of the plants was determined by measuring superoxide dismutase (SOD) and catalase (CAT) activities. On the 8th day of the salt stressed callus culture, growth...

Author(s):   Sebnem Kusvuran, Sebnem Ellialtioglu, Fikret Yasar and Kazim Abak  

January 2012

Augmenting the salt tolerance in wheat (Triticum aestivum) through exogenously applied silicon

  Although silicon improves the salt tolerance in grasses, the mechanism involved is still ambiguous. Moreover in wheat, classified as Si-accumulator, silicon can alleviate the salt stress. Hence in this study, the effects of silicon using calcium silicate (150 mg/L) on the morphology, physiology and biochemistry of wheat genotypes (salt sensitive; Auqab-2000 and salt tolerant; SARC-5) differing in salt...

Author(s):   Anser Ali, Shahzad M. A. Basra, Javaid Iqbal, Safdar Hussain, M. N. Subhani, Muhammad Sarwar and Muhammad Ahmed  

January 2012

Degradation of petroleum hydrocarbons by oil field isolated bacterial consortium

  A mixed consortium was prepared with 15 bacteria isolated by enrichment technique from the sample collected from an oil contaminated site. This consortium was incubated with crude oil to investigate the metabolic capability of bacteria. The degradation efficiency of the isolates in consortium was checked with 2% crude oil by shake flask transformation in mineral salt medium, at 37°C for 24 days. Total...

Author(s):   Zulfiqar Ali Malik and Safia Ahmed  

January 2012

Growth of Enterococcus durans E204 producing bacteriocin-like substance in MRS Broth: Description of the growth and quantification of the bacteriocin-like substance

  Bacteriocin-like substance E204 is an antimicrobial compound produced byEnterococcus durans E204 isolated from camel milk of Morocco that shows a broad spectrum of inhibitory activity against taxonomically related microorganisms. It is sensitive to digestive proteases. In the first study, de Man, Regosa and Sharpe (MRS) broth was inoculated by E204 strain, incubated for 30°C at 200 rpm and monitored...

Author(s):   El Ouardy Khay, Lorenzo Miguel Pastrana Castro, Paula Fajardo Bernárdez, Nadia Skali Senhaji, Mohamed Idaomar and Jamal Abrini

January 2012

Biological activity of Streptomyces noursei against ochratoxin A producing Aspergillus niger

  Aspergillus niger isolated from some common medicinal and herbal plants used in Egypt, was found to be producer for ochratoxin A. This research investigate the biological control of ochratoxin A production by A. niger, using some bacterial isolates belonging to true bacteria (Bacillus pumilus), Lactic acid bacteria (Lactobacillus acidophilus, Lactobacillus...

Author(s):   Nanis G. Allam, Abd El-Raheem R. El-Shanshoury, Hamdy A. Emara and Amira Z. Zaky    

January 2012

Peroxidase isozyme profiles in some sweet cherry rootstocks and ‘0900 Ziraat’ cherry variety

  This study was carried out on one- year old trees of ‘0900 Ziraat’ variety grafted onto KuÅŸ kirazı, Kara idris, Sarı idris, MaxMa 14, MaxMa 60 and Gisela 5 in order to determine their compatibility. For this purpose, peroxidase isoenzyme bands were determined with polyacrylamide gel electrophoresis (PAGE). Saplings were grafted at the beginning of September with ‘T’ budding....

Author(s):   S. F. Güçlü and Fatma Koyuncu  

January 2012

Rapid detection and isolation of Salmonella sp. from amphibians and reptiles

  The principal objective of this study was to assess the presence of Salmonella sp. in imported amphibians and reptiles. A total of 49 samples were collected from commercial pet shops and life science museums in Korea and examined via the traditional detection method (TDM) and the PCR method. Salmonella typhimuriumand Salmonella enteritidis were screened via multiplex PCR (mPCR). The...

Author(s):   Sang Phil Shin, Hyo Jin Yang, Ji Hyung Kim, Casiano H. Choresca Jr., Jee Eun Han, Jin Woo Jun, Sang Yoon Han and Se Chang Park  

January 2012

Evidence for a role of KISS-1/GPR54 system in decreased luteinizing hormone (LH) secretion in fasted prepubertal ewes

  Short fasting has been shown to suppress luteinizing hormone (LH) secretion in ewes, however, the molecular mechanism underlying this phenomenon is not clear. Based on recent discovery of the crucial role of KiSS-1/GPR54 system in the central control of the GnRH axis, we hypothesized that KiSS-1/GPR54 system was involvedin the inhibitory effect of fasting on LH secretion in ewes. To test...

Author(s):   Jun Wang, Haizhu zhou, Tao Zhang, Lei Sun, Jing Xu and Yujie Lou  

January 2012

Characterization of polycaprolactone and starch blends for potential application within the biomaterials field

  Thermally blending starch and polycaprolactone with the compatibilizer methylenediphenyl diisocyanate (MDI) distributed in the polyester phase had been previously confirmed to enhance the mechanical properties and interface between the two phases of the blends. In this study, blends of polycaprolactone with various levels of starch and 1.0% MDI were prepared through melt blending using a twin-screw...

Author(s):   Jianyu Su,Ling Chen and Lin Li  

January 2012

Primary drug resistance patterns in newly diagnosed tuberculosis patients in Yazd, Southern Province of Iran

  Prevalence of multidrug resistant (MDR) and extensively drug-resistant tuberculosis (XDR) has been a cause of concern for tuberculosis (TB) control in both developed and developing countries. The objective of this study was to investigate the prevalence of drug resistance patterns among newly diagnosed tuberculosis cases, in Yazd, the southern province of Iran, during 2009 to 2010. The drug susceptibility...

Author(s):   Sharifi Yazdi M. K., , Jabbari H., Soltan Dallal  M. M, and Bahrmand A.  

January 2012

Effects of RNA interference targeting Smad7 on nerve cells ischemic injury induced in PC12 cells

  Ischemic cerebrovascular disease is a global health problem. According to the World Health Organization, ischemic stroke is actually the most common cause of death in the world. ActA/smads signaling pathways was shown to be required for the differentiation-associated physiological apoptosis of stroke. Although smad7 is an important regulator of ActA/smads signaling via a negative feedback...

Author(s):   Chunli Mei, Jing Mang, Jinting He, Guihua Xu, Zhongshu Li, Wenzhao Liang and Zhongxin Xu 

January 2012

The phenomenon of cross tolerance in osmotically and ionically adapted rice (Oryza sativa L.) cell lines

  In the present study, cell lines of local rice (Oryza sativa L.) cv. Swat-1 were incrementally adapted to osmotic [20% Poly ethylene glycol (PEG)] and ion specific (20 mM LiCl) stresses to investigate the phenomenon of cross tolerance. The tolerances of adapted lines were stable up to six generation on stress free medium. Both adapted cell lines were highly tolerant to either osmotic or ionic...

Author(s):   Azhar H. Shah, Safdar H. Shah, Habib Ahmad, Zahoor A. Swati, Abrar H. Shah, M. Afzal, Umm-e-Aiman and Q. Khalid  

January 2012

Serum biochemical values and mineral contents of tissues in Przewalski’s and Tibetan gazelles

  Reference values were established for some serum hematological and biochemical constituents in Przewaiski’s and Tibetan gazelles in China. The contents of mineral elements in the blood, hair, liver, lung and muscle of Przewaiski’s and Tibetan gazelles were also measured. All values are reported for the first time for Przewaiski’s and Tibetan gazelles. Most hematological...

Author(s):   Xiaoyun Shen,  and Zhigang Jiang