December 2013
Polymorphism, sequencing and phylogenetic characterization of growth differentiation factor 9 (GDF9) gene in Assam Hill goat
Assam hill goat (Capra hircus) is a prolific local goat in India. Growth differentiation factor (GDF9) gene was studied as a candidate gene for the prolificacy of goats. The objective of the present study was to detect the incidence of mutation in the exonic region of GDF9 gene of Assam hill goat. Total number of 90 blood samples were collected randomly from different parts of Assam and genomic DNA were extracted using...
December 2013
Isolation and characterization of partial mitochondrial CO1 gene from harpacticoid copepod, Leptocaris canariensis (Lang, 1965)
Copepods are gaining attention as superior viable live feed for larviculture and as ecological bioindicators. One of the possible candidates from Malaysia is Leptocaris canariensis (Copepoda: Harpacticoida). However, little is known about the molecular aspect of this species. In this study, DNA of individual L. canariensis was extracted and the partial mitochondrial CO1 gene was successfully amplified using universal...
December 2013
Marker assisted characterization of chickpea genotypes for wilt resistance
Marker assisted characterization of six chickpea genotypes differing for Fusarium wilt reaction was carried out using seven molecular markers reported by earlier workers linked to disease resistant/susceptibility. In the present study, four different markers (namely, CS-27, UBC-170, CS-27A and UBC-825) linked to susceptibility and three microsatellite based markers (TA-59, TA-96 and TR-19) linked to resistance allele...
December 2013
DNA polymorphism analysis of Xanthomonas campestris pv. campestris using single-strand conformation polymorphism (SSCP) and random amplified polymorphic DNA (RAPD)
The applicability of the random amplified polymorphic DNA (RAPD) and single-strand conformation polymorphism (SSCP) techniques using M13 and 16S rRNA primers, respectively, for genotyping of the phytopathogenic bacterium Xanthomonas campestris pv. campestris was studied. RAPD provided a simple, rapid, and reliable method to identify genetic variation, and SSCP was used to screen for sequence polymorphisms. A 730-bp...
December 2013
Aphid resistance in florist's chrysanthemum (Chrysanthemum morifolium Ramat.) induced by sea anemone equistatin overexpression
Florist's chrysanthemum (Chrysanthemum morifolium Ramat.) belongs to the Asteraceae family and represents the second most important floricultural crop in the world. Most genotypes are sensitive to aphids and infestations can lower quality and cause transmission of viruses. The protease inhibitor Sea Anemone Equistatin (SAE) carries three domains responsible for the inhibition of both cysteine and aspartic proteases....
December 2013
Adsorption of violet B by agricultural waste of soft pistachio shells
In this study, inexpensive agricultural waste pistachio sells was used for adsorption of violet B. Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) were used to characterize the pistachio shells. The morphology of pistachio shell was studied by SEM and it showed the porous structure of pistachio shells. Different parameters like contact time, pH and mass of sorbent were...
December 2013
Determination of heavy metals and genotoxicity of water from an artesian well in the city of Vazante-MG, Brazil
The city of Vazante-MG is of great socioeconomic and environmental interest because it is the most important zinc producer district of Brazil. The mineral processing and geochemical processes may determine high concentrations of heavy metals in water intended for human consumption. Thus, the present study aimed to quantify and evaluate the heavy metal genotoxicity of artesian water in the city by Atomic absorption...
December 2013
Production of L-glutaminase and its optimization from a novel marine isolate Vibrio azureus JK-79
L-Glutaminase is an amidohydrolase which is produced by a variety of micro-organisms including bacteria, yeast and fungi. It is currently used in the treatment of acute lymphoblastic leukemia (ALL) and human immunodeficiency virus (HIV). Another potential application of L-glutaminase is as flavor enhancing agent in soy sauce fermentation. Even though L-glutaminase activity was reported in numerous micro-organisms,...
December 2013
Aerobic Bacterial degraders in effluent from Itoku textile industry, Abeokuta
The local textile industry in Itokun village is one major textile industry in Abeokuta Ogun state, known for “adire” production whose processes are not maintained at regulatory standards. This study involves isolating and identifying aerobic microorganisms in waste water effluents from this textile Industry and screening for aerobic degraders among the isolates. Fifty milliliter (50 ml) of three dye...
December 2013
Optimization of culture variables for the production of L- asparaginase from Pectobacterium carotovorum
L-Asparaginase is an anti-neoplastic agent used in the chemotherapy of acute lymphoblastic leukemia. The present work deals with production of intra-cellular L-asparaginase from Pectobacterium carotovorum using submerged fermentation. The statistical approaches of Plackett-Burman and response surface methodology were applied to optimize the culture parameters for the production of L-asparaginase through a bacterium P....
December 2013
Influence of alcohol: oil molar ratio on the production of ethyl esters by enzymatic transesterification of canola oil
The influence of alcohol:oil molar ratio on the canola oil transesterification reaction in solvent-free medium using free lipase from Thermomyces lanuginosus and Burkholderia cepacia was studied. The experiments conducted in batch reactor for 72 h at 37°C in cosolvent-free reaction system with ethanol addition in three steps showed great potential for ester production. The stepwise addition of ethanol allowed...
December 2013
Evaluation of anti-hyperglycemic activity and side effects of Erythraea centaurium (L.) Pers. in rats
In folk medicine, several plants, among which the small centaury, are recommended for the treatment of diabetes type 2 in humans. An experimental study to evaluate anti hyperglycemic effect of Erythraea centaurium (L.) Pers. was performed on wistar rats. Normoglycemic rats and rats subjected to oral glucose tolerance test overload “OGTT” were used. Administration to these animals by gavage of 20% aqueous...
December 2013
Association of plasma protein C levels and coronary artery disease in men
Several studies have shown the risk factor causes of coronary heart disease. In this study we tested the hypothesis that plasma protein C level might be used as a biomarker for coronary heart disease and myocardial infarction. The study included 60 men that were classified into 3 groups according to clinical examination; group I set as healthy control group, group II set as patients with ischemic heart disease and group...
December 2013
Emerging Acinetobacter schindleri in red eye infection of Pangasius sutchi
This communication provides an insight into the emerging of new infection “red eye” in Pangasius sutchi and aimed to screen the prime pathogens involved in disease. The pathogen was isolated from diseased P. sutchi and characterized by morphological, biochemical and molecular approach, which includes 16s r RNA gene sequencing. Polymerase chain reaction (PCR) amplified 16s RNA was separated using agarose gel...
December 2013
Isolation and characterisation of Listeria species from ruminants in Maiduguri north–eastern Nigeria
A cross sectional study was carried out to determine the prevalence of Listeria species in ruminants in Maiduguri. Three hundred faecal samples were randomly collected from ruminants at the Maiduguri central abattoir from January – March, 2011. One hundred faecal samples each were collected from cattle, sheep and goat at ante mortem by balloting comprising of fifty samples each from male and female animals. Forty...
December 2013
Biochemical characterization of indigenous Fulani and Yoruba ecotypes chicken of Nigeria
The study was carried out to characterize two indigenous chickens of Nigeria using protein markers; haemolglobin (HB) and carbonic anhydrase (CA). Separation of the two proteins was achieved by cellulose acetate electrophoresis and direct gene counting method was employed to interpret the result. Palentological statistics was used to generate dendrogram that measured genetic similarity within and between each of the...
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