African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 11 October 2012; 11(82)

October 2012

Assessment of genetic diversity in wheat spring genotypes by molecular markers in northern Iran

  Genetic diversity of spring wheat cultivars has very important role in reducing genetic vulnerability during plant breeding and crossing programs. Molecular markers are main tools for identifying of the genetic diversity. In this study, simple sequence repeat (SSR) and random amplified polymorphic deoxyribonucleic acid (RAPD) markers were used to determine the genetic diversity of 10 bread wheat genotypes...

Author(s):   Khavarinejad, M. S. and Karimov, M.  

October 2012

Phylogenetic relationships in genus Gloriosa L.

  In an attempt to test the monophyly of the genus Gloriosa L., the chloroplast regiontrnL-trnF sequences were employed using Baeometra uniflora, Hexacyrtis dickiana,Neodregea glassii, Onixotis punctata, Onixotis stricta, Ornithoglossum parviflorum,Ornithoglossum undulatum, Ornithoglossum viride, Ornithoglossum vulgare andSandersonia aurantiaca as an...

Author(s):   Alfred Maroyi  

October 2012

Genetic polymorphism of five genes associated with growth traits in goat

  Genetic polymorphism studies in domestic animals aim at evaluating genetic variations within and across breeds mainly for conservation purposes. In this study, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect polymorphisms of five candidate genes in four Egyptian and Saudi goat breeds (Barki, Zaribi, Ardi and Masri), to detect the genotype of GH, IGF1,...

Author(s):   Saleha Y. M. Alakilli, Karima F. Mahrous, Lamiaa M. Salem, and Ekram S. Ahmed  

October 2012

Optimization of microsatellite DNA Gelred fluorescence imaging technology

  Gelred fluorescent dye has broader prospects of application in DNA experiment because of its high sensitivity, security and stability. In order to explore the best microsatellite DNA Gelred imaging technology, this study compared its dosage by using three methods; precasting gels method (PG), staining sample method (SS) and immersion gels method (IG). The results show that agarose gel electrophoresis (AGE)...

Author(s):   Qin Yue, Fu Da-li, Liu Meng-pei, Liang Yu-qin and Wu Shuo  

October 2012

Evaluation of the sensitivity of two plantain varieties essong and big ebanga to the nematode Radopholus similis

  Plantains are among the crops that enhance food security in Cameroon. However, plantain yields remain low because of several constraints of parasites such asRadopholus similis, a nematode that can cause losses up to 75% in fields when no protection measures are taken. In most cases, the sensitivity of local plantain varieties to R. similis is unknown; which generally results to low yields despite...

Author(s):   Philippe Kosma,, Ambang Zachée, Begoude Dider, Ten Hoopen Martijn, Kuate Jean and Amougou Akoa  

October 2012

Recloning of regenerated plantlets from elite oil palm (Elaeis guineensis Jacq.) cv. Tenera

  Plant regeneration in oil palm cv. Tenera via somatic embryogenesis was conducted using regenerated plantlets as an explant source. Explants from different positions – apex, middle and basal segments of regenerated plantlets – were cultured in N6 medium supplemented with 100, 120 and 140 mg/L 2,4-dichlorophenoxyacetic acid(2,4-D) in the presence of activated charcoal. The production of...

Author(s):   Mya Thuzar, Apichart Vanavichit, Somvong Tragoonrung and Chatchawan Jantasuriyarat,  

October 2012

In vitro propagation and organogenesis of Lilium ‘Prato’

  Lilium consists of more than 80 species native to the Northern Hemisphere. It is widely used as cut flowers, flowering potted and garden plants. Since Lilium in vitroproduction, as an alternative to the conventional vegetative propagation methods is becoming an important way to increase shoot proliferation rates; therefore, the purpose of this study was to establish a protocol for in...

Author(s):   Hany El-Naggar, Amira Osman and Eman Sewedan  

October 2012

Assessment of genetic relationships among Spring Dendrobium cultivars and varietal materials using amplified fragment length polymorphism (AFLP) analysis

  (AFLP) markers with near-infrared fluorescence-labeled primers. Eight EcoRI + 3 bases/MseI + 3 bases primer set combinations were used in this investigation. Each selected primer set generated 113 to 158 scorable fragments. A total of 1102 AFLP fragments were detected, of which 778 were polymorphic (70.6%). An unweighted pair-group method of the arithmetic averages (UPGMA), principal coordinate analysis...

Author(s):   Zheng Quan, Zheng Yongping, Guo Weiming, Lin Weijun and Wang Guangdong  

October 2012

Effects of day-night supplemental UV-A on growth, photosynthetic pigments and antioxidant system of pea seedlings in glasshouse

  A glasshouse experiment was carried out to investigate the effects of day-night supplemental UV-A on growth, photosynthetic pigments and antioxidant system (contents of antioxidant substances and antioxidant enzyme activity) of pea seedlings substrate-cultivated in glasshouse. The results show that supplemental UV-A did not significantly affect shoot and root to shoot ratio of pea seedlings, but root and...

Author(s):   Liu Wenke, and Yang Qichang,  

October 2012

Influence of different soaking times with selenium on growth, metabolic activities of wheat seedlings under low temperature stress

  Low temperature (LT) is one of the most important factors limiting the growth, development and distribution of wheat plants in temperate regions. Selenium often acts as an antioxidant in plants and this study hypothesize that selenium application can partly alleviate LT-induced oxidative stress and negative impacts of LT on wheat (Triticum aestivum L.) plant. Wheat seeds were soaked in aqueous...

Author(s):   Samia Ageeb Akladious  

October 2012

Uptake and elimination kinetics of heavy metals by earthworm (Eudrilus eugenia) exposed to used engine oil-contaminated soil

  Earthworm inoculation of petroleum hydrocarbon contaminated soil is thought to catalyze the bioremediation. Most bioremediation studies focus on the petroleum hydrocarbon content and not on the heavy metals. Here, the uptake kinetics of heavy metals by earthworm in used engine oil contaminated soil was investigated. The metals considered were zinc (Zn), copper (Cu), nickel (Ni), chromium (Cr), titanium (Ti)...

Author(s):   Alewo Opuada AMEH, Ibrahim Ali MOHAMMED-DABO, Sani IBRAHIM, Joseph Baba AMEH Saidu Muhammad WAZIRI, Josephine Onyama ODENGLE, Tajudeen Kolawole BELLO and Yahuza TANIMU  

October 2012

Biomodification of palm shell activated carbon using Aspergillus niger and Bacillus subtilis and its effect on the adsorption of lead ions from aqueous solutions

  Adsorption of lead ions from aqueous solutions using commercial untreated granular palm shell activated carbon (PSAC) and PSAC biomodified with Bacillus subtilis andAspergillus niger biomass, respectively, was studied. The adsorption capacity of the three biosorbents was evaluated in batch adsorption experiments at pH 3 to 6; lead concentration varied between 20 to 300 mg/L. The PSAC-B....

Author(s):   Issabayeva Gulnaziya, Aroua Mohd Kheireddine and Chen Siew Kim  

October 2012

In vitro screening of fungicides and antagonists against Sclerotium rolfsii

  A study was conducted in the microbiology laboratory of Plant Pathology Department, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, during 2010 to 2011 to control Sclerotium rolfsii with fungicides and Trichoderma harzianum. Six fungicides namely Provax-200, Bavistin, Ridomil, Dithane M-45, Rovral 50 WP and Tilt were screened at 100, 200 and 400 ppm concentration for their...

Author(s):   Bhuiyan, M. A. H. B., Rahman, M. T. and Bhuiyan, K. A.  

October 2012

Secondary metabolism responses in two Pisum sativum L. cultivars cultivated under Fe deficiency conditions

  The present study was carried out to investigate the Fe deficiency effect on the secondary metabolism responses in two Pisum sativum cultivars characterized by different tolerance to Fe deficiency. Previous study investigating the physiological responses to Fe deficiency in these two pea cultivars showed that Kelvedon was more tolerant than Lincoln. Both cultivars were grown in the...

Author(s):   Nahida JELALI#, Smiri MOEZ#, Wissal DHIFI, Wissem MNIF, Chedly ABDELLY and Mohamed GHARSALLI    

October 2012

Biochemical response and host-pathogen relation of stalk rot fungi in early stages of maize (Zea mays L.)

  Stalk rot is a destructive disease in maize caused by Fusarium and Macrophominaspecies. A study was carried out to understand the mode of infection, host biochemical response and comparison of inoculation techniques in Fusarium verticillioides and Macrophomina phaseolina in maize. In seed inoculation experiment, high mycelia growth on seed surface lead to rotting in 36.6% of...

Author(s):   Nirupma Singh, Ambika Rajendran, Meena Shekhar and Girish Mittal  

October 2012

Antibacterial, antioxidant and cytotoxic activities of extracts from the thermophilic green alga, Cosmarium sp.

  Unicellular green algal strains, identified as Cosmarium (Chlorophyta), were isolated from Aïn-Echeffa hot spring in north Tunisia. Different extracts (methanol, hexane, acetone, acetone: methanol and water) obtained from both biomass and extracellular polysaccharides (EPS) were evaluated for their antibacterial, antioxidant and cytotoxic activities. First, extracts were tested in...

Author(s):   Rafika CHALLOUF, Rym BEN DHIEB, Héla OMRANE, Khémissa GHOZZI and Hatem BEN OUADA