African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Table of Content: 11 September 2013; 12(37)

September 2013

The role of biofortification in the reduction of micronutrient food insecurity in developing countries

Micronutrient malnutrition is a global public health problem, especially in developing countries. Hunger and starvation which are causative agents of malnutrition are occasioned by poor food supply and low income purchasing power for the expensive animal sources of micronutrients. Access to adequate, safe and nutritious food required for a healthy and active life by all people at all times is limited, resulting in...

Author(s): Uchendu  Florence Ngozi

September 2013

Genetic differentiation and inheritance of random amplified polymorphic DNA (RAPD) markers in pectoral spine phenotypic sub-groups of Clarias gariepinus

Information on genetic relationship of phenotypically divergent sub-groups would be useful for better identification, utilization and management of species. Recent study revealed phenotypic divergence in a reservoir population of Clarias gariepinus. Genetic variability of polymerase chain reaction (PCR) products of the phenotypic divergent sub-groups was investigated in this study. Polymorphism and genetic...

Author(s): O. O. Oyebola, B. O. Omitoyin, A. E. Salako, E. K. Ajani and M. O. Awodiran

September 2013

Establishment of core collection from apricot germplasm in China

This study aimed at establishing a core collection based on the analysis of data from Simple Sequence Repeat (SSR) alleles and morphological and agronomical traits (MOR) of the primary core collection from apricot germplasm resources. The index of genetic diversity, and frequency ratios of retention and loss of the alleles were studied between cluster and random sampling methods at 5 sampling ratios. The results...

Author(s): Zhang Jun Huan, Wang Yu Zhu, Sun Hao yuan, Yang Li and Ning Ning

September 2013

Effects of coagulating enzyme types (commercial calf rennet, Aspergillus niger var. awamori as recombinant chymosin and rhizomucor miehei as microbial rennet) on the chemical and sensory characteristics of white pickled cheese

The possibilities of using recombinant chymosin as an alternative coagulant to commercial calf rennet in the production of white pickled cheese was investigated. For this purpose, white pickled cheese produced by using commercial calf rennet, recombinant chymosin (Aspergillus niger var. awamori) and microbial rennet (Rhizomucor miehei) were compared in terms of their chemical and organoleptic properties. The...

Author(s): Fatma ÇepoÄŸlu and Mutlu B. Güler-Akın

September 2013

Development of polyclonal antibodies for the detection of recombinant human erythropoietin

Recombinant human erythropoietin (rHuEPO) is detected by using direct pharmacological assays and indirect haematological assays. However, both methods have several limitations including technical challenges and cost-related issues. The aim of this study was to develop polyclonal antibodies against rHuEPO (anti-rHuEPO pAb) that can be used in immunoassays. In this study, we purified anti-rHuEPO pAb that could be used in...

Author(s): Collares Thaís Farias, Monte Leonardo Garcia, Campos Vinicius Farias, Seixas Fabiana Kömmiling, Collares Tiago Veiras, Dellagostin Odir and Hartleben Cláudia Pinho

September 2013

Alteration in lipid metabolism induced by a diet rich in soya-oil and amylopectin in a rat model

This present study was designed to evaluate the impact of different dietary regimens on lipid metabolism in brain, liver and plasma of albino rat model. Twenty (20) male Wister albino rats (110 g) were assigned to two dietary groups and housed individually. One group received a control diet enriched in 20% soy- oil and another enriched with 20% amylopectin. The starved group was fed primarily with carboxyl methyl...

Author(s): Adejoke N. Kolawole, Theo L. Olawoye and Ayodele O. Kolawole

September 2013

Anti-motility and reductions in the concentrations of gut electrolytes: Mechanisms for the anti-spasmodic use of the seeds of avocado (Persea americana Mill) in folk medicine

The seeds of avocado (Persea americana Mill) are used in traditional medicine to treat, allay or prevent some spasm-related disorders, for instance, diarrhoea. The chloroform and methanol fractions of the chloroform-methanol extract of the seeds ofP. americana were investigated for their qualitative and quantitative phytochemical constituents as well as effects on gastro-intestinal motility (transit) and...

Author(s): ODO Christian E., NWODO Okwesili F.C., JOSHUA Parker E., OMEH Yusuf S., OKONKWO Christopher C., GOMETI Ajiroghene S. , NWEJE-ANYALOWU Paul C. and DICKSON Dickson I.

September 2013

Economic feasibility of on-farm fuel ethanol production from cassava tubers in rural communities

A simple process for on-farm bioethanol production from cassava, using cassava kojisupplemented with crude liquid enzyme and yeast was described. On a small scale, a fed-batch mode where 4 kg of koji, 2 kg of gelatinized cassava flour and 30 g of yeast cells were mixed and allowed to ferment for 2 days, followed by addition of 1.5 kg of cassava flour and fermenting for another 3 days, gave higher ethanol...

Author(s): Christiana N. Ogbonna and Eric C. Okoli