African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 13 December 2012; 11(100)

December 2012

An assessment of genetic fidelity of in vitro grown plantlets of rose (Rosa hybrida) through molecular markers

  A simple and routine method for the analysis of somaclonal variation among tissue culture derived rose plants is a prerequisite for precise monitoring of quality control during rapid mass micropropagation. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) molecular marker techniques were employed to validate the genetic fidelity in three varieties of Rosa...

Author(s): S. K. SENAPATI, S. APARAJITA and G. R. ROUT

December 2012

Genetic diversity of Ardi goat based on microsatellite analysis

  The aim of this study was to analyze the genetic variability of Ardi goats found in the central regions of the kingdom of Saudi Arabia using 14 microsatellite markers. Allelic richness was considerably high in this population indicating high genetic polymorphism as expected heterozygozity was 0.675. Furthermore, the population showed deviation from Hardy-Weinberg equilibrium in seven loci. Mean polymorphic...

Author(s): Riyadh S. Aljumaah,, Muneeb M. Musthafa, Mohamed A. Al-Shaikh, Osama M. Badri and Mansour F. Hussein

December 2012

Prevalence, incidence and molecular identification of root-knot nematodes of tomato in Pakistan

  Tomato is a widely grown vegetable in Pakistan. However, its production is severely constrained by root knot nematodes (RKNs). Accurate identification of RKNs is essential for an appropriate control program. The current study evaluated the prevalence, incidence and diversity of RKNs of tomato crops grown in the Khyber Pakhtunkhwa Province and their identification using molecular tools. A field survey,...

Author(s):   Ishrat Naz, Juan E. Palomares-Rius, Vivian Blok, Saifullah, Sardar Ali and Musharraf Ahmed

December 2012

Identification of avian Mycoplasma species in commercial broilers and layers with respiratory symptoms in Balochistan

  Among many avian mycoplasmas, only the Mycoplasmas gallisepticum (MG) andMycoplasmas synoviae (MS) are responsible for causing respiratory disease in commercial poultry. This study reported for the very first time the serological occurrence of M. gallisepticum and M. synoviae in blood samples (n = 600) from sixty flocks (n = 42 broiler and n = 18 layer...

Author(s): M. A. Atique, F. Abbas, M. A. Awan, M. M. Tariq, Z. Ahmed, Irshad Ali and M. A. Alam

December 2012

Domestic wastewater treated for agricultural reuse

  Four post-treatment systems of an upflow anaerobic sludge blanket (UASB) reactor were evaluated regarding chemical and sanitary qualities. At an experimental station for biological treatment of sewage (EXTRABES) located in Campina Grande - PB, Brazil, the physical, chemical and biological parameters of the effluents from a UASB reactor and its post-treatment systems were monitored for two years. A baffled...

Author(s): José Tavares de Sousa, Rita de Cássia Vieira Alves, Wilton da Silva  Lopes, Valderi Duarte Leite  and José Lima de Oliveira Júnior

December 2012

The properties and interaction of auxins and cytokinins influence rooting of shoot cultures of Eucalyptus

  Success in Eucalyptus micropropagation varies with genotype. Although some protocols have proven suitable for suites of clones, many genotypes are recalcitrant to rooting. Their micropropagation is addressed empirically through the manipulation of auxins and cytokinins, which work antagonistically to produce roots and shoots, respectively. Rooting success of three genotypes with 0.1 mg/l...

Author(s): Muhammad Nakhooda, M. Paula Watt and David Mycock

December 2012

Protection of melon plants against Cucumber mosaic virus infection using Pseudomonas fluorescens biofertilizer

  This study was carried out to characterize a virus causing severe mosaic, yellowing, stunting and leaf deformation on melon (Cucumis melo L.), and evaluate the capacity of Pseudomonas fluorescens as biofertilizer to improve plant growth and restrict the accumulation of the virus in the plant. The virus was identified as an isolate ofCucumber mosaic virus (CMV) by means of symptoms on...

Author(s): Rakib A. Al-Ani and Mustafa A. Adhab

December 2012

Experimental biogas research by anaerobic digestion of waste of animal origin and biodegradable garden waste

  Currently, one of the most efficient and prospective methods of biodegradable waste management is anaerobic digestion in a bio-reactor. The use of this method for managing biodegradable waste generating in agriculture and elsewhere would result in the recovery of biogas that could be used as an alternative to natural gas. The article presents the results of the experimental tests of biogas production through...

Author(s): Alvydas Zagorskis, Pranas Baltrėnas, and Antonas Misevičius

December 2012

Isolation and identification of bacterial strain I33M producing milk-clotting enzyme: Optimization of culture parameters using response surface

  A strain I33M which produces a milk-clotting enzyme was screened from Algerian soil near a dairy factory. This strain was identified as Bacillus mojavensis based on morphology and internal transcription spacer sequence. Sequencing analysis of 16S rDNA gene showed 100% identity of the tested strain with the B. mojavensis in the database. Phylogenetic analysis of this strain showed that...

Author(s): Talantikite-Kellil, S, Nouani, A. , Saunier, M, Gillmann, L, Brahmi, A and Fazouane, F

December 2012

Enhanced sporulation and toxin production by a mutant derivative of Bacillus thuringiensis

  Bacillus thuringiensis (Bt) strains along with insecticidal crystal proteins (ICP) also produce proteases, and chitinases during growth and sporulation. These both potentate the activity of ICPs. This work aimed to obtain bioinsecticide over-production and thermotolerant mutant through classical mutagenesis of vegetative cells of Bt by using UV-mutation multiple times. The isolated survivors...

Author(s): Muhammad Ibrahim Rajoka, Fatima Jalal,  and Shazia A. Bukhari

December 2012

Whole body and egg amino acid composition of Nile perch, Lates niloticus (Linnaeus, 1758) and prediction of its dietary essential amino acid requirements

  Eggs and tissue of Nile perch juveniles were analysed in order to derive the essential amino acid dietary requirements for Nile perch larvae and juveniles, using the A/E ratio. Results revealed the presence of both essential and non-essential amino acids in Nile perch. A significant difference (p < 0.05) between the amino acids (AA) composition in the eggs and tissue and amongst the four class sizes of...

Author(s): Namulawa, V. T., Rutaisire, J. and Britz, P. J.

December 2012

Saccharification of delignified sawdust from 20 different trees in the Lagos area of Nigeria

  Sawdust produced during the chopping of trees is a major waste product causing pollution of air as well as of the Lagos Lagoon in Nigeria. Sawdust from 20 different trees processed in the wood industry has been delignified successively by the Kraft process and hydrogen peroxide followed by Trichoderma viride cellulose catalyzed bioconversion into glucose, a fermentable sugar. Sugars are also...

Author(s): N. A. Ndukwe, W. O. Okiei, B. I. Alo, J. P. H. van Wyk, T. M. Mamaboloand C. C. Igwe