African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Table of Content: 13 November, 2013; 12(46)

November 2013

A review on trypanosomosis in dogs and cats

Trypanosoma brucei brucei, Trypanosoma cruzi and Trypanosoma congolense were initially thought to be the only species of trypanosomes capable of causing diseases in dogs and cats. However, dogs and cats are challenged by diverse species of trypanosomes with varying virulence and pathogenicity. Dogs may develop clinical trypanosomosis by infection with Trypanosoma evansi but are refractory to Trypanosoma rangeli of man....

Author(s): Nwoha, R. I. O.

November 2013

Comparative effectiveness of inter-simple sequence repeat and randomly amplified polymorphic DNA markers to study genetic diversity of Indian Garcinia

A study to compare the effectiveness of inter-simple sequence repeats (ISSR) and randomly amplified polymorphic DNA (RAPD) profiling was carried out with a total of 65 DNA samples using 12 species of Indian Garcinia. ISSR and RAPD profiling were performed with 19 and 12 primers, respectively. ISSR markers generated a total number of 156 bands with 92 polymorphic bands, while RAPD markers produced a total of 134 bands...

Author(s): Utpala Parthasarathy, O. P. Nandakishore, K. Nirmal babu, Senthil Kumar and V. A. Parthasarathy

November 2013

Sensory evaluation of different preparations of cassava leaves from three species as a leafy vegetable

Cassava leaves are largely consumed in Africa and are among the top three African indigenous vegetables rich in nutrients. Leaves from bitter (Manihot utilissima), sweet (Manihot dulcis) and wild (Manihot glaziovii) species of cassava were cooked by boiling in salted (sodium bicarbonate and table salt) water with the addition of palm oil and ground-nut paste, following processing  by “pounding”,...

Author(s): M. G. Umuhozariho, , N. B. Shayo, P. Y. K. Sallah and J. M. Msuya

November 2013

Evaluation of pollen viability, stigma receptivity and fertilization success in Lagerstroemia indica L.

To provide theoretical basis for artificial pollination in Lagerstroemia indica L., pollen viability and stigma receptivity were tested and the morphological change of stigma was observed. Pollen viability tested by in vitro culture, stigma receptivity examined by benzidine-H2O2 testing and fruit set estimated by field artificial pollination were analyzed in this study. The maximum pollen viability was observed at 10:00...

Author(s): Fang Chen, Wei Yuan, XuePing Shi and YaoMei Ye*

November 2013

Micropropagation of Helianthemum lippii L. var Sessiliforuim (Cistaceae) an important pastoral plant of North African arid areas

Helianthemum lippii L. is a perennial, little brush distributed in sandy regions of southern Tunisia as well as of Mediterranean basin. It presents an important ecological, economical and pastoral interest. All these characteristics encourage us to try the in vitro propagation because it is a feasible alternative for the rapid multiplication and the preservation of plants. A very high frequency of sprouting and shoot...

Author(s): Amina Hamza, Maher Gtari and Neffati Mohamed

November 2013

Partial dehydration of 'Niagara Rosada' GRAPES (Vitis labrusca L.) targeting increased concentration of phenolic compounds and soluble solids

The partial dehydration of grapes after harvest and aimed wine-making, has been shown to be a process that brings increased concentration of sugar and phenolic compounds in the must, which results in the quality of the wines produced. However, the works developed so far studied the process for temperatures up to a maximum of 25°C and air velocity less than 1 m.s-1. This study aimed to analyze the physical and...

Author(s): Wesley Esdras Santiago, Rodolpho César Dos Reis Tinini, Rafael Augustos De Oliveira and Barbara Janet Teruel

November 2013

Preliminary screening of plant essential oils against larvae of Culex quinquefasciatus Say (Diptera: Culicidae)

Preliminary screenings of 22 plant essential oils were tested for mortality of the mosquito larvae Culex quinquefasciatus under laboratory conditions. Percent (%) mortality of the mosquito larvae were obtained for each essential oil.  At different exposure periods, viz. 1, 3, 6, 12 and 24 h among the 22 plant oils tested, eight oils viz., aniseed, calamus, cinnamon, clove, lemon, orange, thyme, and tulsi oils gave...

Author(s): M. Ramar, M. Gabriel Paulraj and S. Ignacimuthu

November 2013

The role of seed priming in improving seed germination and seedling growth of maize (Zea mays L.) under salt stress at laboratory conditions

Salinity is considered as a major abiotic stress affecting germination, seedling growth and crop production in arid and semi-arid regions. Many techniques are used to improve tolerance to salinity. Priming is believed to be an effective technique that increases germination, plant growth and improve yield of several vegetables and crops under saline soil condition. The objective of this study was to see the effect of...

Author(s): Gebremedhn Yohannes and Berhanu Abraha

November 2013

Pathogenicity of Beauveria bassiana and production of cuticle-degrading enzymes in the presence of Diatraea saccharalis cuticle

The sugarcane borer, Diatraea saccharalis, is one of the worst pests in Brazilian sugarcane crop, causing high levels of financial losses every year. Beauveria bassiana is an entomopathogenic fungus widely used in the biological control of several agricultural pests. The aims of this study were to: (1) evaluate the pathogenicity of  B. bassiana strains against  D. saccharalis  (2) investigate the...

Author(s): Virgínia Michelle Svedese, Patricia Vieira Tiago, Jadson Diogo Pereira Bezerra, Laura Mesquita Paiva, Elza Áurea de Luna Alves Lima, Ana Lúcia Figueiredo Porto

November 2013

A comparative study of pectinolytic enzyme production by Bacillus species

The economic and ecological function of pectinase enzymes in industries is gaining much attention with the need of highly productive strains of microorganisms to reduce production cost. The present investigation is a comparative evaluation of Bacillus sp. pectinases. The three isolates isolated from agro-waste, Bacillus stearothermophilus, Bacillus cereus and Bacillus subtilis, showed very high pectinase activities with...

Author(s): Torimiro N. and Okonji, R. E.

November 2013

Bio-corrosion of water pipeline by sulphate-reducing bacteria in a mining environment

Detrimental effects of corrosion in conjunction with bacterial activity in water pipeline systems have led to an increase in research in how to prevent such occurrences. In particular, sulphate-reducing bacteria (SRB), found in anaerobic conditions underneath the main corrosion shell, are noteworthy for their effects in promoting localized corrosion. This study investigates the presence of SRB in water, in a water...

Author(s): Z. Manafi, M. Hashemi, H. Abdollahi and Gregory. J. Olson

November 2013

An investigation of the bioaccumulation of chromium and uranium metals by Cynodon dactylon: A case study of abandoned New Union Gold Mine Tailings, Limpopo, South Africa

Mine waste, including tailings is generally outlined as one of the largest environmental concern which faces defunct mines in South Africa and New Union Gold Mine is no exception. These tailing contain heavy metal such as chromium (Cr) and uranium (U) which poses enormous threat to the environment even at small quantity. The study focuses mainly on bioaccumulation of Cr and U in soil by Cynodon dactylon, an indigenous...

Author(s): K. Nelushi, J. R. Gumbo and F. A. Dacosta

November 2013

In vitro cultivation of Pleurotus ostreatus and Lentinula edodes in lignocellulosic residues from Amazon

The mycelial growth speed of Pleurotus ostreatus (POS 09/100) and Lentinula edodes (LED 96/13) was evaluated in four substrates based on Simarouba amara sawdust, Anacardium giganteum sawdust, Euterpe precatoria seeds and Musa sp. AAB stems. The loss of organic matter of the substrates and the mycelial vigor of the strains studied were also evaluated. The greatest mycelial growth for P. ostreatus occurred in the...

Author(s): Ceci Sales-Campos, Diego A. Pires, Samira R. L. Barbosa, Raimunda Liége S. Abreu and Meire C. N. Andrade

November 2013

Second derivative spectrophotometric determination of cyclophosphamide in pharmaceutical formulations

A new, simple, rapid, wide applicable range and reliable second derivative spectrophotometric method has been developed for determination of cyclophosphamide (CP) in bulk and pharmaceutical dosage forms. Calibration graph is linear in the concentration range of 25 - 200 μg/ml of CP with 10 μg/ml of detection limit and correlation coefficient of 0.9976. The precision and accuracy were acceptable depending upon the...

Author(s): Nabil A. Fakhre, Hemn A. Qader and Alaadin M. Naqishbandi

November 2013

Effects of hypoxia on serum hepatic chemistries of Tibet chicken and Shouguang chicken

Hypoxia is a major factor that affects the subsistence and development of multicellular organisms. Tibet chicken, as a unique native chicken breed in altiplano, shows genetic adaptation to hypoxia comparing with the breeds at the low altitude. In the present study, to explore effects of hypoxia on chicken fetal livers, eggs of Tibet chicken and Shouguang chicken were collected and the samples from each breed were...

Author(s): Wenpeng Han#, Meiling Song,#, Hui Yuan#, Haigang Bao, Chong Liu, Changxin Wu and Chunjiang Zhao