African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 13 September 2012; 11(74)

September 2012

Molecular characterization of capsid protein gene of potato virus X from Pakistan

Potato (Solanum tuberosum L.) is one of the most economically important vegetable crops in Pakistan. Chlorotic thickness veins spots intermingled with a dark green area, mosaic and decrease in size of the leaves were observed in the Lahore during a survey in 2009. Reverse transcriptase polymerase chain reaction (RT-PCR) based detection conditions were optimized for potato virus X using specific primers...

Author(s): Arshad Jamal, Idrees Ahmad Nasir, Bushra Tabassum, Muhammad Tariq,Abdul Munim Farooq, Zahida Qamar, Mohsin Ahmad Khan, Nadeem Ahmad, Muhammad Shafiq, Muhammad Saleem Haider, M. Arshad Javed, and Tayyab Husnain

September 2012

Construction of genetic map in barley using sequence-related amplified polymorphism markers, a new molecular marker technique

Sequence-related amplified polymorphism (SRAP) markers, a novel polymerase chain reaction (PCR)-based molecular marker technique, were successfully applied in map construction, cultivar identification, diversity evaluation, comparative genomics and gene location of different plant species. The molecular genetic map of SRAP markers in Steptoe / Morex doubled haploid (DH) population was constructed in this study, using...

Author(s): Leilei Guo#, Xianjun Liu#, Xinchun Liu, Zhimin Yang, Deyuan Kong, Yujing He and Zongyun Feng#

September 2012

Relationship between morphological and amplified fragment length polymorphism (AFLP) marker based genetic distance with heterosis in hot pepper (Capsicum annuum L.)

Identification of potential parents that produce the hybrids with superior yield is the most important step in developing hybrids to save the substantial resources. The present study was carried out to assess the morphological and amplified fragment length polymorphism (AFLP) marker based genetic diversity, to estimate mid-parent heterosis and to correlate the estimated parental genetic diversity with heterosis chilli....

Author(s): Krishnamurthy S. L., Mohan Rao A., Madhavi Reddy K., Ramesh S., Shailaja Hittalmani and Gopinath Rao M.

September 2012

Isolation of a novel abscisic acid stress ripening (OsASR) gene from rice and analysis of the response of this gene to abiotic stresses

Abiotic stresses constitute a serious threat to agricultural production, which often develops into major crop production reducing factors around the world. Molecular biology technology has, however, emerged as a promising vehicle improving crop tolerance. A cold-, drought- and heat-inducible gene designated Oryza sativa L. abscisic acid stress-ripening (OsASR) gene, GenBank accession: AK318549.1 was...

Author(s): Rongjun Chen, Jiali Dong, Shengbin Liu, Zhengjun Xu and Xiaoling Gao

September 2012

The effect of different sowing patterns and deficit irrigation management on yield and agronomic characteristics of sweet corn

Water stress restricts crop yields in both the arid and semi-arid zones of the world. The responses of sweet corn (Zea mays) to irrigation frequency and sowing patterns were studied in the field from December 2005 to December 2006. This research was laid out in split plot, with water quantity as main plot and sowing patterns as subplot in three replications. The treatments consisted of two irrigation times (6 and 10...

Author(s): Saberi, A. R., Kiani, A. R., Mosavat, S. A. and Halim, R. A.

September 2012

A comparison of the toxicity of the botanical insecticide, Sirinol and two chemical insecticides, Mospilan and Consult, on two natural enemies of the pistachio psyllid, coccinellid predator (Oenopia conglobata) and parasitic wasp (Psyllaephagus pistaciae)

The common pistachio psyllid, Agonoscena pistaciae, is one of the most important pests of pistachio tree. This pest has many natural enemies and some of the important ones are the coccinellid predator (CP) (Oenopia conglobata) and the parasitic wasp (PW) (Psyllaephagus pistaciae). In this research, the effect of three concentrations of Sirinol on the first and fourth instar larvae and the adult of O....

Author(s): Kabiri, M., Amiri-Besheli, B. and Basirat, M.

September 2012

Correlation between resistance of eggplant and defense-related enzymes and biochemical substances of leaves

14 eggplant cultivars were inoculated by Verticillium dahliae to screen their resistance against verticillium wilt. The resistances were shown as the disease incidence and disease index, and eggplant cultivars were classified into resistant type (R), moderate resistant type (MR), tolerant type (T), moderate susceptible (MS) and susceptible type (S), according to the final disease index. To find out the...

Author(s): Baoli Zhou, Zhixia Chen, Liang Du, Xueling Ye and Ning Li

September 2012

Reducing ammonia volatilization from compound fertilizers amended with zeolite

Ammonia volatilization is a direct loss of available nitrogen in agriculture. The objective of this study was to determine the effect of amending NPK fertilizer with different rates of clinoptilolite zeolite on ammonia volatilization, soil exchangeable ammonium, and available nitrate. Seven treatments evaluated were: 250 g soil alone (T1), 3.28 g ammonium nitrate (AN) without additive (T2), 44.6 g 5:3:2 formulated...

Author(s): W. B. M. Lija, O. H. Ahmed, and S. Kasim

September 2012

Cloning and characterization of a g-tocopherol methyltransferase from Chinese cabbage

α-Tocopherol, with antioxidant properties, is synthesized by photosynthetic organisms and play important roles in human and animal nutrition. The final step of the α-tocopherol biosynthetic pathway is catalyzed by g-tocopherol methyltransferase (g-TMT). In the major oilseed crops, g-tocopherol, the biosynthetic precursor of α-tocopherol, is the predominant form. The full-length cDNA...

Author(s): Vu Hai Tran, Wei Wang and Dong-Zhi Wei

September 2012

Antimicrobial activity of some potential active compounds against food spoilage microorganisms

Antimicrobial activities of six potential active compounds (acetic acid, chitosan, catechin, gallic acid, lysozyme, and nisin) at the concentration of 500 mg/ml against the growth of Escherichia coli, Staphylococcus aureus, Listeria innocua, andSaccharomyces cerevisiae were determined. Lysozyme showed the highest antimicrobial activity against L. innocua and S. cerevisiae with an...

Author(s): Saroat Rawdkuen, Phunsiri Suthiluk, Damrongpol Kamhangwong and Soottawat Benjakul

September 2012

Inhibition of fruit infestation by Mediterranean fruit fly using natural products

The inhibitory effects of some bio-products on the infestation of Ceratitis capitataWied were studied. Fully ripe fruits of red mombin (Spondias purpurea L.) were cleaned, dried and treated with the products: Proagrim, essential oils of fennel (Foeniculum vulgare Mill.) and orange (Citrus sinensis L.), at concentrations of 0.0 (control), 1.0, 1.5, 2.0, 2.5 and 3.0% (w/v). The results...

Author(s): Flávia Queiroz de Oliveira, José Bruno Malaquias, Wênnia Rafaelly de Souza Figueiredo, Jacinto de Luna Batista and Eduardo Barbosa Beserra