African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 14 August, 2012; 11(65)

August 2012

Phytochemistry and pharmacologic properties of Myristica fragrans Hoyutt.: A review

  Myristica fragrans is known as “nutmeg”, its extracts and essential oil are important in drug development with numerous pharmacological activities in South Africa, India and other tropical countries. For a long time, M. fragrans has been used in traditional medicines as a carminative, stimulant, narcotic, emmenagogue and abortifacient. Nutmeg is also prescribed for the treatment of...

Author(s):   Jinous Asgarpanah and Nastaran Kazemivash  

August 2012

Mapping of randomly amplified polymorphic DNA primer (RAPD) on chromosome 2A of common wheat

  Randomly amplified polymorphic DNA primers (RAPDs) were mapped on chromosomes 2A of wheat genotypes using “Chinese Spring” nullisomic-tetrasomic lines. One particular pair of chromosome was absent and another homologous pair was present in the extra dose. Genomic DNA was isolated from two genetic stocks viz; NT1D1B and NT2A2B. Polymerase chain reaction (PCR) were conducted using RAPD primers...

Author(s):   Khamsa Parveen, Inamullah, Habib Ahmad, Muhammad Sajjad Iqbal, Fida MuhammadAbbassi, Aziz-ud-Din, Abdullah Khan and Imtiaz Ahmad Khan    

August 2012

Population genetic data for six non-combined DNA index system (non-CODIS) miniSTR loci from the Xhosa and Cape Muslim populations of South Africa

  Allele frequency and forensic parameters for six non-combined DNA Index System(non-CODIS) miniSTR loci: D1S1627, D3S4529, D5S2500, D6S1017, D8S1115, and D9S2157 were calculated from a sample of 226 unrelated individuals from two populations (that is, Xhosa and Cape Muslim) of South Africa. No significant deviation from Hardy-Weinberg equilibrium was found. All loci showed a moderate degree of...

Author(s):   Zainonesa Abrahams and Mongi Benjeddou    

August 2012

cDNA cloning of v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT) in silver fox (Vulpes vulpes) and its sequence polymorphism analysis among foxes and dogs

  As a critical receptor for basic cell functions, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT) has been found to be closely related to many types of tumors and coat colour in animals. In this study, we cloned and sequenced the full-length coding sequence of KIT in silver fox (Vulpes vulpes). Alignment together with the public accessible mRNA sequences of dogs and...

Author(s):   Chunyan Bai, Jiaxin Yu, Wei Zhao, Jiani Hou, Yumei Li and Shouqing Yan  

August 2012

Genetic variation among Northern and Southern Egyptian buffaloes using polymerase chain reaction-random amplified polymorphic DNA (PCR-RAPD)

  The domestic water buffalo is a species of great economic potential, especially in developing countries like Egypt. Egyptian buffalo have been classified according to minor phenotypic differences and their geographical locations.  Few studies have taken place to investigate the genetic variations in Egyptian buffalo using microsatellites analysis. In the present study, 11 random primers were analyzed for...

Author(s):   Othman E. Othman, Eman R. Mahfouz and Soheir M. El Nahas  

August 2012

In vitro propagation of Acacia hybrid through alginate-encapsulated shoots and axillary buds

  Seed collected from Acacia hybrid trees yields highly variable and poorly performing offspring and are not commonly used in regeneration. The present study described the encapsulation of Acacia hybrid shoots and axillary buds in the calcium alginate gel. The aim of the study was to evaluate the germination of the buds in vitro on the medium with different concentrations of plant...

Author(s):   Nor Asmah H., Nor Hasnida H., Noraliza A., Nashatul Zaimah N. A. and Nadiah Salmi N.    

August 2012

Effects of organic manures on yield, fruit quality, nutrients and heavy metals content of Barhy date palm cultivar

  A field experiment was conducted to investigate the effects of plant residues and/or inorganic manures with or without chemical fertilizers (NPK) on yield, fruit quality and contents of some nutrients and heavy metals of fruit of Barhy date palm cultivar during 2009 to 2010 and 2010 to 2011 in Jouff, Saudi Arabia. The following five treatments were used in this experiment: agricultural waste + mineral...

Author(s):   S. H. Al-Kahtani and S. S. Soliman    

August 2012

Improving formulated nitrogen, phosphorus and potassium compound fertilizer using zeolite

  Ammonia volatilization from urea and ammonium containing compound fertilizers cause higher cost of fertilization in agriculture. In this study, an incubation experiment was carried out to compare the effect of different ratios of compound fertilizer amended with clinoptilolite zeolite on NH3 volatilization, soil exchangeable NH4 and available NO3 contents on Bekenu Series (Tipik Tualemkuts)...

Author(s):   K. A. Rabai, O. H. Ahmed and S. Kasim  

August 2012

Construction of a molecular marker linkage map and its use for quantitative trait locus (QTLs) underlying drought tolerance at germination stage in soybean

  A backcross inbred line (BIL) population of soybean was examined under polyethylene glycol (PEG) and well-watered conditions to identify the quantitative trait locus (QTL) controlling the drought-tolerance at germination stage. The recipient, SNWS0048, was a wild soybean with strong drought tolerance and the donor, Jinda73, was a drought-sensitive variety with superior agronomic traits....

Author(s):   Min WANG, Wan-ming YANG and Wei-jun DU  

August 2012

An overview of genomics assisted improvement of drought tolerance in maize (Zea mays L.): QTL approaches

  Today drought is the worldwide problem that causes the reduction in the crop yield of plants. Maize (Zea mays L.) is greatly affected by drought and its production is declining day by day. However there are many techniques accessible today to achieve a better understanding of the multifarious network of plant responses to drought and to maneuver these responses. These techniques comprise the association...

Author(s):   Qurban Ali, Muhammad Ahsan, Muhammad Hammad Nadeem Tahir, Ihsan Khaliq, Muhammad Kashif, Mehboob Elahi, Jehanzab Farooq, Nazar Hussain Khan, Muhammad Tahir Naveed, Fawad Ali, Usman Saeed and Muhammad Anwar  

August 2012

Expression and subcellular localization of antiporter regulating protein OsARP in rice induced by submergence, salt and drought stresses

  We examined the expression and subcellular localization of antiporter regulating protein OsARPin a submergence tolerant rice (Oryza sativa L.) cultivar FR13A. In the public databases, this protein was designated as putative Os02g0465900 protein. The cDNA containing the full-length sequence of OsARP gene was present in Gene Bank Accession no. AK071205 and this encoded 216 amino acids which had...

Author(s):   Md Imtiaz Uddin, Maki Kihara, Lina Yin, Mst Farida Perveen and Kiyoshi Tanaka  

August 2012

Screening of Lactobacillus plantarum isolated from fermented idli batter for probiotic properties

  Probiotics are defined as live microorganisms which when administered in adequate amounts confer a health benefit on the host. The objective of this study was to screen eight potential probiotic Lactobacillus plantarum strains from fermented idli batter using in vitro assays such as bile tolerance, acid tolerance, transit tolerance in the upper human gastrointestinal tract,...

Author(s):   Perumal Jayaprabha Agaliya and Kadirvelu Jeevaratnam  

August 2012

Isolation and screening of microorganisms from a gari fermentation process for starter culture development

  Cassava (Manihot esculenta Crantz), is used for the production of a variety of West African foods and ranks fourth in the list of major crops in developing countries after rice, wheat and maize. Gari is one of the most popular foods produced from cassava. Cassava may contain high levels of linamarin, a cyanogenic glucoside, which in its natural state is toxic to man. Therefore, some processing...

Author(s):   Vinodh A. Edward,, Moutairou Egounlety, Melanie Huch, Petrus J. Van Zyl, Suren Singh, Naledzani D. Nesengani, Vetja M. Haakuria, Charles M.A.P. Franz  

August 2012

The effect of Acacia karroo supplementation and thermal preparation on meat sensory characteristics of the indigenous Xhosa lop-eared goat genotype

  Seed collected from Acacia hybrid trees yields highly variable and poorly performing offspring and are not commonly used in regeneration. The present study described the encapsulation of Acacia hybrid shoots and axillary buds in the calcium alginate gel. The aim of the study was to evaluate the germination of the buds in vitro on the medium with different concentrations of plant...

Author(s):   S. Ngambu, V. Muchenje and U. Marume,    

August 2012

Purification, composition analysis and antioxidant activity of different polysaccharides from the fruiting bodies of Pholiota adiposa

  Three water-soluble polysaccharide fractions (PAP1-1, PAP1-2 and PAP2-1) were isolated and purified from the fruiting bodies of Pholiota adiposa by diethylaminoethanol (DEAE) Sepharose fast flow column and superdexTM 200 column chromatography on an ÄKTA Purifier system. Results of high-performance size-exclusion chromatography (HPSEC) indicated that the average molecular weights of PAP1-1,...

Author(s):   Yongxin Nie, Hongxia Jiang, Yanyou Su, Changxiang Zhu, Jiarui Li and Fujiang Wen      

August 2012

Simplex and triplex polymerase chain reaction (PCR) for identification of three medically important Candida species

  Candida species are a major cause of invasive infections in both critically ill and immunocompromised patients. Hence, rapid identification of these pathogens may facilitate specific therapy and patient management. The development of rapid and specific diagnostic methods remains a challenge. Herein, we developed the simplex and triplex polymerase chain reaction (PCR) for the identification of three...

Author(s):   Nabil S. Harmal, Alireza Khodavandi, Mohammed A. Alshawsh, Farida Jamal, Zamberi Sekawi, Ng Kee Peng and Pei Pei Chong    

August 2012

Inhibiting Aspergillus flavus growth and degrading aflatoxin B1 by combined beneficial microbes

  Aflatoxin B1 (AFB1) is a type of toxin produced by Aspergillus flavus, which has a negative effect on animal production and economic profits. In order to inhibit A. flavusgrowth and degrade aflatoxin, the optimal proportion of beneficial microbes such asLactobacillus casei, Bacillus subtilis and Pichia anomala were selected. The results show that AFB1 production and...

Author(s):   Ruiyu Zuo, Juan Chang, Qingqiang Yin, Ping Wang, Wei Cheng, Xiao Wang, Junxi Liu and Qiuhong Zheng      

August 2012

Bioassay guided isolation and identification of anti-inflammatory and anti-microbial compounds from Urtica dioica L. (Urticaceae) leaves

  The present study describes the anti-inflammatory, anti-microbial activity and lipophilic profile with acute toxicological studies of Urtica dioica. Successive extraction of the leaves with organic solvents of increasing polarity and their screening for anti-inflammatory and anti-microbial activity was assessed. Hexane extract showed good anti-inflammatory and anti-microbial activity; hence it was...

Author(s):   Sabzar Ahmad Dar, A. R. Yousuf, Farooq Ahmad Ganai, Poonam Sharma, Naresh Kumar and Rambir Singh      

August 2012

The essential oil composition of Carthamus tinctorius L. flowers growing in Iran

  The composition of the essential oil obtained from the dried flowers of Carthamus tinctorius L. growing in Iran was analyzed by gas chromatography (GC) and gas chromatography-mass spectrophotometry (GC-MS). 29 compounds were identified in the oil. The major compounds of the oil were 1-hydroxy-3-propyl-5-(4-methyl-penten)-2-methylbenzene (25.2%), 2,5,5 trimethyl-3-propyl,tetra hydro 1- naphtol...

Author(s):   Parisa Ziarati, Jinous Asgarpanah and Mehrnaz Kianifard    

August 2012

Enhancement of the feeding value of wheat offal for broiler feeding after its solid state fermentation with Aspergillus niger

  The objective of this study was to investigate the changes in in vitro dry matter digestibility (IVDMD) of wheat offal (WTO) after its biodegradation with Aspergillus niger and also to determine the effect of degraded WTO on the performance parameters of broiler birds at starter and finisher phases. A. niger was used for the biodegradation of WTO. There were five dietary...

Author(s):   T.E Lawal, G.F Faniyi, O.M Alabi, S.G Ademola and T. O Lawal