African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 14 June 2012; 11(48)

June 2012

Enhancing the biological nitrogen fixation of leguminous crops grown under stressed environments

Legumes have the ability to establish a symbiotic interaction with soil bacteria, collectively termed as rhizobia. These bacteria can enhance growth and developmentof associated crops by transferring atmospheric nitrogen into a form that is available for plant growth or by improving nutrient uptake through modulation of hormone-linked phenomena in inoculated plants. Selection of the...

Author(s): Yassine Mabrouk and Omrane Belhadj

June 2012

Teratogenicity and brain aromatase-induction of monosodium glutamate in estrogen-responsive mosaic transgenic zebra fish Danio rerio

Monosodium glutamate (MSG) has been used as a flavor enhancer for decades. It has various teratogenicity effects on tested animals but has not been examined in zebra fish model to date. This experiment was conducted to study the teratogenic effects of MSG on wild-type zebra fish embryos and also to study the estrogenic potential of MSG on the transient zebrafish embryos with a brain aromatase-based reporter gene....

Author(s): Tamer Said Abdelkader, Chang Seo-Na, Kim Tae-Hyun, Song Juha, Kim dongso  and Jae-Hak Park

June 2012

Cloning and expression analysis of potassium channel gene NKT3 from Nicotiana tabacum

Potassium (K+) is the predominant inorganic ion of plant cells. K+ channels in higher plant cells play an important role in regulating the influx and efflux of K+ from cells, and activity of these channels might be involved in plant stress resistance. A completely new K+ channel gene of Nicotiana tabacum was obtained through homologous cloning strategy. The complete cDNA sequence was...

Author(s): Haobao Liu, Pingzhi Qu, Guanshan Liu, Yuhe Sun, Qian Wang, Yirong Jin, Yufeng Song and Ming Zhao

June 2012

Development and characterization of microsatellite loci for Fenneropenaeus penicillatus Alcock

Eight novel microsatellite loci from the genome of Fenneropenaeus penicillatus Alcock were developed using the protocol of fast isolation by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Thirty (30) wild individuals were used to analyze the polymorphism of these eight microsatellite markers. The results show that the number of alleles per locus and the polymorphism...

Author(s): Yuanyu Cao, Zhongbao Li, Qinghui Li, Xiaojiao Chen, Lina Chen and Gang Dai

June 2012

Effect of polymer and irrigation regimes on dry matter yield and several physiological traits of forage sorghum

Drought stress is one of the most serious problems in crops production in the arid and semi-arid regions. Application of some materials such as superab A200 polymers (SAP) in soil can improve soil water reservation. They can also decrease water losses, fertilizer leaching in soils, effects of water deficiency in plants and so can improve the yield in the arid and semi-arid regions. This experiment was conducted on...

Author(s): M. Fazeli Rostampour, M. Yarnia and F. Rahimzadeh Khoee

June 2012

In vitro regeneration, detection of somaclonal variation and screening for mosaic virus in sugarcane (Saccharum spp.) somaclones

Three sugarcane accessions susceptible to sugarcane mosaic virus; HSF-240, S-2000-US-359, and S-2003-US-704 were evaluated for callogenesis and regeneration ability. For callogenesis, five different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) was used. The best callogenesis was obtained when Murashige and Skoog (MS) was portified with 3 mg/L...

Author(s): Smiullah, Farooq Ahmad Khan, Abdullah, Aqeel Afzal, M. Aslam Javed, Zafar Iqbal Rameez Iftikhar and Javed Iqbal Wattoo

June 2012

Boron, zinc and microtone effects on growth, chlorophyll contents and yield attributes in rice (Oryza sativa L.) cultivar

The micronutrient required in minute quantity and their deficiency leads to diminished growth and yield of crops. The effect of soil application of Zinc (Zn), Boron (B) and Microtone (MT) on growth, chlorophyll contents, mineral profile and yield components were investigated in rice at Chakkanwali Reclamation Research Station District, Gujranwala, Pakistan in the year 2011. Seven treatments which includes Zn...

Author(s): Muhammad Arif, Muhammad Asif Shehzad, Fiaz Bashir, Muhammad Tasneem, Ghulam Yasin and Munawar Iqbal

June 2012

Inter-populations genetic and morphological diversity in three Silene (Caryophyllaceae) species

The inter-populations morphological and genetic variations were studied in three species of Silene (Silene indeprensa, Silene gynodioica and Silene crispans) of the section Auriculatae, which grow and form several populations in different regions of Iran. Morphological analysis of variance (ANOVA) and analysis of molecular (AMOVA) analyses showed the species...

Author(s): Masoud Sheidai, Roza Eftekharian, Zahra Noormohammadi and Abbas Gholipoor

June 2012

Evaluation of the bacteriological and physicochemical quality of water supplies in Nsukka, Southeast, Nigeria

The bacteriological and physicochemical quality of various water samples from bore hole, dug well and spring, collected from ten different locations within Nsukka were determined. Total viable and coliform counts were evaluated using the standard plate count method and the most probable number (MPN) technique, respectively. The physicochemical parameters were analyzed using standard methods. The mean total bacteria...

Author(s): Anyanwu, C. U. and Okoli, E. N.

June 2012

Morphological and cytosine DNA methylation changes induced by a combined effect of boron (B) and salt toxicity in Sorghum bicolor inbred line

Boron (B) toxicity is one of the abiotic stresses limiting plant growth in arid and semi arid regions globally. Although studies have been conducted on the combined effect of B and sodium chloride (NaCl) toxicity on overall plant growth revealing an antagonistic relationship, the morphology and epigenetic interactions have not fully been explained. Germinating seeds of an inbred line of Sorghum bicolor (YN267)...

Author(s): Rebecca S. Agboola, , Josphert N. Kimatu,Yu-Cai Liao and Bao Liu

June 2012

Comparative study of potato cultivation through micropropagation and conventional farming methods

A trial was carried out to evaluate the productivity of Solanum tuberosum L. cultivated through conventional farming and micropropagation method. Survival rate, biomass and tuber yield of both micropropagated and tuber propagated potatoes was evaluated. Survival percentages of potatoes were 90% for conventional propagation and 85% for micropropagation. The survival rate of micropropagated plants were...

Author(s): P. Singh, R. K. Agnihotri, S. Bhadauria, Rashmi Vamil and R. Sharma

June 2012

In vitro propagation of Rosa hybrida L. cv. Al-Taif Rose plant

In this study, a protocol for in vitro propagation of Rosa hybrida L. cv. Al-Taif Rose was established using nodal segments harboring axillary buds as explants. In vitro stages of shoot initiation, multiplication and elongation were performed. Explants were cultured on solid Murashige and Skoog medium (MS) supplemented with different concentrations of benzyl aminopurine (BAP, 1, 2 and 3...

Author(s): Attia O. Attia, EL Dessoky S. Dessoky, and Adel E. El-Tarras

June 2012

Exposure to crude microcystins via intraperitoneal injection, but not oral gavage, causes hepatotoxicity in ducks

Recently, large-scale cyanobacterial blooms have occurred in fishponds near the suburbs of Xinxiang City in China. The present study aimed to identify the cyanobacterial blooms in the fishpond and evaluate their toxicity on ducks via intraperitoneal injection or oral exposure (gavage) of crude microcystins obtained from the scum of cyanobacterial bloom. The results of the acute toxicity tests showed...

Author(s): Xiao-Yu Li, Shan-Hu Wang, Chun-Yu Wang, Xiao Bai and Jun-Guo Ma

June 2012

Effect of mineral and organic fertilizers on the growth and calyx yield of roselle (Hibiscus sabdariffa L.)

Yield study was carried out during the 2006 summer season in the field of Research Institute, University of Zabol at Sistan (latitude 30°54’ N and longitude 61°41’E). The goal of the study was to evaluate the reaction of roselle cultivar to different chemical fertilizers alone or in combination with organic manure. Ten fertilization treatments were used for this study (mineral fertilizer, hen manure,...

Author(s): Mehdi Dahmardeh

June 2012

Acute and sub-chronic oral toxicity studies of the extracts from herbs in Phikud Navakot

This study presents data from oral acute and sub-chronic (90-day) toxicity effect of the extracts from herbs in Phikud Navakot (PN) which is commonly used in Thai traditional medicine for alleviation of the clinical sign of hyperlipidemia, cardiovascular diseases, and cerebrovascular diseases. The single and repeated dose of the extracts were administered to Sprague Dawley rats as described in the OECD code 420...

Author(s): K. Kengkoom, K. Chaimongkolnukul, S. Cherdyu, R. Inpunkaew and S. Ampawong

June 2012

Identification of the forensically important flies (Diptera: Muscidae) based on cytochrome oxidase subunit I (COI) gene in China

Accurate species identification is a crucial step in forensic entomology, as the insect collected on a corpse can provide useful information for estimation of postmortem interval (PMI). However, morphological distinction may on occasion be impossible to the adult flies and nymphs of the same genus. DNA-based method can be used as a supplemental means of morphological method. In this study, 31 forensically important...

Author(s): Feng Xiong, Yadong Guo, Baihua Luo, Lin Zhang, Jifeng Cai, Xiaolong Li and Zhen Yang

June 2012

Nigella sativa provides protection against metabolic syndrome

The seeds of Nigella sativa have been used in folk medicine all over the world. The plant has been of interest due to its low degree of toxicity and beneficial pharmacological properties like antihypertensive, hypoglycemic, antifungal, anti-inflammatory, antihistaminic, antioxidant, along with significant anti-neuplastic activities. The present clinical study was undertaken to ascertain the adjuvant effect...

Author(s): Abdul Saboor Shah, Gul Majid Khan, Amir Badshah, Shefaat Ullah Shah, Kifayat Ullah Shah, Shakeel Ahmad Mirza, Kifayat Ullah Shah and Kamran Ahmad khan